Information on EC 1.2.1.11 - aspartate-semialdehyde dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.2.1.11
-
RECOMMENDED NAME
GeneOntology No.
aspartate-semialdehyde dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
Phosphorylation
-
-
-
-
redox reaction
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ectoine biosynthesis
-
-
grixazone biosynthesis
-
-
L-homoserine biosynthesis
-
-
L-lysine biosynthesis I
-
-
L-lysine biosynthesis II
-
-
L-lysine biosynthesis III
-
-
L-lysine biosynthesis VI
-
-
norspermidine biosynthesis
-
-
spermidine biosynthesis II
-
-
threonine metabolism
-
-
Glycine, serine and threonine metabolism
-
-
Monobactam biosynthesis
-
-
Cysteine and methionine metabolism
-
-
Lysine biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Microbial metabolism in diverse environments
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
L-aspartate-4-semialdehyde:NADP+ oxidoreductase (phosphorylating)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9000-98-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
wild type strain G165-3A and mutant strain G184-1A
-
-
Manually annotated by BRENDA team
no activity in Homo sapiens
methicillin resistant
-
-
Manually annotated by BRENDA team
methicillin resistant
-
-
Manually annotated by BRENDA team
gene asd
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aspartate-4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH + H+
show the reaction diagram
beta-3-methylaspartyl phosphate + NADPH
beta-3-methylaspartate 4-semialdehyde + phosphate + NADP+
show the reaction diagram
-
-
-
?
L-4-aspartyl phosphate + NADPH
L-aspartate 4-semialdehyde + phosphate + NADP+
show the reaction diagram
L-4-aspartyl phosphate + NADPH
L-aspartate-4-semialdehyde + phosphate + NADP+
show the reaction diagram
L-4-aspartyl phosphate + NADPH + H+
L-aspartate 4-semialdehyde + phosphate + NADP+
show the reaction diagram
L-aspartate 4-semialdehyde + phosphate + NAD+
L-4-aspartyl phosphate + NADH + H+
show the reaction diagram
L-aspartate 4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH
show the reaction diagram
L-aspartate 4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH + H+
show the reaction diagram
L-aspartate-4-semialdehyde + cacodylate + NADP+
L-4-aspartyl cacodylate + NADPH
show the reaction diagram
10% of the activity with phosphate
-
-
r
L-aspartate-4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
aspartate-4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH + H+
show the reaction diagram
L-4-aspartyl phosphate + NADPH
L-aspartate 4-semialdehyde + phosphate + NADP+
show the reaction diagram
L-4-aspartyl phosphate + NADPH
L-aspartate-4-semialdehyde + phosphate + NADP+
show the reaction diagram
L-4-aspartyl phosphate + NADPH + H+
L-aspartate 4-semialdehyde + phosphate + NADP+
show the reaction diagram
L-aspartate 4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH + H+
show the reaction diagram
L-aspartate-4-semialdehyde + phosphate + NADP+
L-4-aspartyl phosphate + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R)-5-[(2-carboxyphenyl)carbamoyl]cyclohexa-3,5-diene-1,3-dicarboxylate
(1R)-5-[(3-nitrophenyl)carbamoyl]cyclohexa-3,5-diene-1,3-dicarboxylate
(1R)-5-[(4-nitrophenyl)carbamoyl]cyclohexa-3,5-diene-1,3-dicarboxylate
(2R,3aR)-5-[(methylsulfonyl)methyl]-2,3,3a,4-tetrahydro-1H-indole-2-carboxylate
(2R,5R)-2,3,4,5-tetrahydropyridine-2,5-dicarboxylate
(2R,5R)-5-nitro-2,3,4,5-tetrahydropyridine-2-carboxylate
(2R,7aR)-2,3,7,7a-tetrahydro-1H-indole-2,6-dicarboxylate
(2R,7aR)-6-hydroxy-2,3,7,7a-tetrahydro-1H-indole-2-carboxylate
(2R,7aR)-6-nitro-2,3,7,7a-tetrahydro-1H-indole-2-carboxylate
(2R,8aR)-2,7-dinitro-1,2,8,8a-tetrahydronaphthalene
(3aR)-2-oxo-2,3,3a,4-tetrahydro-1H-benzimidazole-5-carboxylic acid
(3aR)-5-nitro-1,3,3a,4-tetrahydro-2H-benzimidazol-2-one
(3aR)-5-nitro-3a,4-dihydro-1H-indene-1,3(2H)-dione
(3aR)-5-nitro-3a,4-dihydro-1H-isoindole-1,3(2H)-dione
(3aR)-6-chloro-5-nitro-3a,4-dihydro-1H-isoindole-1,3(2H)-dione
(7aR)-2-oxo-2,3,7,7a-tetrahydro-1H-indole-6-carboxylate
(7aR)-3-(carboxylatomethyl)-6-nitro-7,7a-dihydro-1H-indole-2-carboxylate
(S)-2-amino-5-fluoro-4-oxo-5-phosphono-pentanoic acid
-
irreversible inhibition
(S)-2-amino-5-phosphono-pent-4-ynoic acid
-
-
2'-phosphoribose AMP
-
-
2-Aminoadipate
3-Chloroacetylpyridine-adenine dinucleotide phosphate
-
NADP+ and NADPH protect
3-hydroxyaspartic acid
-
-
4-([[(1S)-1-carboxy-2-hydroxyethyl]amino]methyl)benzene-1,2-dicarboxylic acid
4-([[(1S)-1-carboxyethyl]amino]methyl)benzene-1,2-dicarboxylic acid
4-nitro-N,N-diethylbenzimidazolinone
4-nitro-N,N-dimethylbenzimidazolinone
4-nitro-N-ethylphthalimide
4-nitro-N-methylphthalimide
5-(carboxylatocarbonyl)-1H-pyrrole-2-carboxylate
5-[[(4-nitrophenyl)amino]carbonyl]-1,3-benzenedimethylcarboxylate
acetylenic and z-olefinic analogues
-
competitive inhibition
-
Adenosine 5'-triphosphate
-
causes a time-dependent inactivation at a concentration of 3.5 mM, 0°C, pH 6.5 and 2 mM dithiothreitol, inactivation can be completely reversed by warming the reaction mixture to 25°C, 50% inactivation occurs at a concentration of 2.5 mM, NADH protects
aromatic aldehydes
-
e.g.: benzaldehyde, weak
caulerpin
-
molecular docking and dynamics simulation of Vibrio anguillarum aspartate semialdehyde dehydrogenase with natural product caulerpin, which binds with high energy. Caulerpin can be used as antibiotic against Vibrio anguillarum in fish aquaculture industry
-
cis-5-phosphonic acid pipecolic acid
-
-
cysteine
-
in the reverse reaction
D-Cystine
-
70% inhibition at 0.01 mM, binds via the cysteine moiety covalently to the catalytic Cys135 of the enzyme, pH-dependent proces, optimal at pH 7.0-7.5, inhibition is reversible by DTT, dithioerythritol, 2-mercaptoethanol, dimercaptopropanol, and reduced glutathione, no protection by aspartate-beta-semialdehyde, NADP+ or NADPH, inhibition mechanism and kinetics
dimethyl pyridine-2,5-dicarboxylate
DTNB
-
reversible by DTT, dithioerythritol, 2-mercaptoethanol, dimercaptopropanol, and reduced glutathione
formaldehyde
-
-
Glutaraldehyde
-
-
GSSG
-
oxidized glutathione
homocysteine
-
in the reverse reaction
iodoacetamide
-
at 0.1 mM: 45.4% inactivation in the absence of NADP+, 22% inactivation in the presence of 1 mM NADP+
iodoacetate
L-2-Amino-4-oxo-5-chloropentanoic acid
L-cystine
L-cystine diethyl ester
-
68% inhibition at 0.01 mM, reversible by DTT, dithioerythritol, 2-mercaptoethanol, dimercaptopropanol, and reduced glutathione
L-cystine dimethyl ester
-
67% inhibition at 0.01 mM, reversible by DTT, dithioerythritol, 2-mercaptoethanol, dimercaptopropanol, and reduced glutathione
L-cystine hydroxamate
-
20% inhibition at 0.01 mM, reversible by DTT, dithioerythritol, 2-mercaptoethanol, dimercaptopropanol, and reduced glutathione
L-isoleucine
L-leucine
-
inhibits, when added to a final concentration of 10 mM in the assay system produces a decrease of 0.004 units in specific activity
L-lysine
L-methionine
L-threonine
methyl 5-nitropyridine-2-carboxylate
N-((4-(2-benzyl)vinyl)benzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(1-naphthyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(2-bromobenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(2-methylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(2-naphthyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(2-trifluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(2-trifluoromethylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(3-bromobenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(3-methylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(3-trifluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(3-trifluoromethylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-(2-perfluoropropyl))-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-biphenyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-bromobenzyl)-N-(2-carboxy)ethyl-3,4-dicarboxybenzylamine
-
-
N-(4-bromobenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-carboxamidebenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-carboxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-difluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-methylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-t-butylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-trifluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(4-trifluoromethylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-(ethylmorpholino)-N-carboxymethyl-3,4-dicarboxybenzylamine
N-acetal-N-carboxymethyl-3,4-dicarboxybenzylamine
N-acetonitrile, N-carboxymethyl-3,4-dicarboxybenzylamine
N-allyl, N-carboxymethyl-3,4-dicarboxybenzylamine
N-benzyl, N-carboxymethyl-3,4-dicarboxybenzylamine
N-carboxyethyl-3,4-dicarboxybenzylamine
N-carboxymethyl-3,4-dicarboxybenzylamine
N-ethylmaleimide
N-methyl, N-carboxymethyl-3,4-dicarboxybenzylamine
NADPH-Tris-chloride buffer
-
promotes a weak inactivation at 0°C, NADH protects
p-hydroxymercuribenzoate
-
-
Periodate
-
-
perrhenate
-
very weak inhibitor
-
petrosamine B
phosphonate
-
weak inhibitor
pipecolic acid-5-(R)-phosphate hydrochloric acid
-
-
pipecolic acid-5-(S)-phosphate hydrochloric acid
-
-
potassium phosphate
-
at a concentration of 10 mM promotes 60% inactivation at 0°C in the presence of 10 mM ATP, at a concentration of 100 mM promotes 61% inactivation in the presence of 10 mM ATP and 32% inactivation in the absence of ATP, NADH protects
S-methyl cysteine sulfoxide
-
inhibitor binding structure deduced from crystal structure
S-methyl-L-cysteine sulfoxide
covalently binding inhibitor via Cys134 at the active site, inactivation, inhibition and binding mechanism, reversible by addition of DTT or 2-mercaptoethanol
tellurate
-
-
thieno[2,3-b]thiophene-2,5-dicarboxylate
trans-5-phosphonic acid pipecolic acid
-
-
Tris salts
tungstate
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
H2CO3
-
enhances activity 4 or 5 times in both directions
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.6
arsenate
-
-
140
cacodylate
-
recombinant wild-type enzyme, pH 9.0, 30°C
6
HAsO42-
-
-
0.16
L-4-aspartyl phosphate
0.12 - 2.6
L-aspartate 4-semialdehyde
0.03 - 0.955
L-aspartate-4-semialdehyde
1.7 - 11.4
NAD+
0.0026 - 1.6
NADP+
0.02 - 3.3
NADPH
0.032 - 240
phosphate
0.14
vanadate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.6
aspartyl phosphate
-
0.12 - 330
L-aspartate 4-semialdehyde
0.24 - 330
L-aspartate-4-semialdehyde
2
L-aspartate-semialdehyde
-
-
9.5 - 115.2
NAD+
3.2 - 330
NADP+
3.2 - 330
phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
236
L-aspartate 4-semialdehyde
-
in 0.2 M bicine buffer, pH 8.6, at 25°C
0.8 - 53.3
NAD+
1124.9 - 9612.8
NADP+
265
phosphate
-
in 0.2 M bicine buffer, pH 8.6, at 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11 - 0.18
(1R)-5-[(4-nitrophenyl)carbamoyl]cyclohexa-3,5-diene-1,3-dicarboxylate
0.69 - 1.2
(2R,5R)-2,3,4,5-tetrahydropyridine-2,5-dicarboxylate
1.2 - 2.1
(2R,5R)-5-nitro-2,3,4,5-tetrahydropyridine-2-carboxylate
0.4 - 1.1
(3aR)-5-nitro-1,3,3a,4-tetrahydro-2H-benzimidazol-2-one
2.6 - 3.3
(3aR)-5-nitro-3a,4-dihydro-1H-isoindole-1,3(2H)-dione
0.15 - 0.18
(3aR)-6-chloro-5-nitro-3a,4-dihydro-1H-isoindole-1,3(2H)-dione
1.2
(S)-2-amino-5-fluoro-4-oxo-5-phosphono-pentanoic acid
-
0.2 M Tris, 1 mM EDTA, pH 8.6, 15 mM phosphate, 0.15 mM NADP+, 37°C
3.9
(S)-2-amino-5-phosphono-pent-4-ynoic acid
-
0.2 M Tris, 1 mM EDTA, pH 8.6, 15 mM phosphate, 0.15 mM NADP+, 37°C
0.05
2'-phosphoribose AMP
-
-
0.04
3-Chloroacetylpyridine-adenine dinucleotide phosphate
-
competitive inhibitor with respect to NADP+
0.324 - 0.654
4-([[(1S)-1-carboxy-2-hydroxyethyl]amino]methyl)benzene-1,2-dicarboxylic acid
0.296 - 0.609
4-([[(1S)-1-carboxyethyl]amino]methyl)benzene-1,2-dicarboxylic acid
4
4-nitro-N,N-diethylbenzimidazolinone
0.086
4-nitro-N,N-dimethylbenzimidazolinone
4
4-nitro-N-ethylphthalimide
0.89 - 1.1
4-nitro-N-methylphthalimide
20
5-[[(4-nitrophenyl)amino]carbonyl]-1,3-benzenedimethylcarboxylate
20
dimethyl pyridine-2,5-dicarboxylate
10
homocysteine
-
-
20
methyl 5-nitropyridine-2-carboxylate
0.476 - 1.1
N-((4-(2-benzyl)vinyl)benzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.396 - 0.749
N-(1-naphthyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.529 - 0.737
N-(2-bromobenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.524 - 0.721
N-(2-methylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.329 - 0.724
N-(2-naphthyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.54 - 0.708
N-(2-trifluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.509 - 0.727
N-(2-trifluoromethylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.442 - 0.665
N-(3-bromobenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.43 - 0.69
N-(3-methylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.427 - 0.684
N-(3-trifluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.441 - 0.678
N-(3-trifluoromethylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.009 - 0.639
N-(4-(2-perfluoropropyl))-N-carboxymethyl-3,4-dicarboxybenzylamine
0.012 - 0.634
N-(4-biphenyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
3.6
N-(4-bromobenzyl)-N-(2-carboxy)ethyl-3,4-dicarboxybenzylamine
-
pH 8.6, 22°C
0.057 - 0.629
N-(4-bromobenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.229 - 0.649
N-(4-carboxamidebenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.072 - 0.635
N-(4-carboxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.054 - 0.698
N-(4-difluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.063 - 0.608
N-(4-methylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.016 - 0.648
N-(4-t-butylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.036 - 0.663
N-(4-trifluoromethoxybenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.024 - 3.8
N-(4-trifluoromethylbenzyl)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.176 - 0.692
N-(ethylmorpholino)-N-carboxymethyl-3,4-dicarboxybenzylamine
0.276 - 0.655
N-acetal-N-carboxymethyl-3,4-dicarboxybenzylamine
0.22 - 0.498
N-acetonitrile, N-carboxymethyl-3,4-dicarboxybenzylamine
0.303 - 0.663
N-allyl, N-carboxymethyl-3,4-dicarboxybenzylamine
0.297 - 0.696
N-benzyl, N-carboxymethyl-3,4-dicarboxybenzylamine
2.4
N-carboxyethyl-3,4-dicarboxybenzylamine
-
pH 8.6, 22°C
0.246 - 0.528
N-carboxymethyl-3,4-dicarboxybenzylamine
0.296 - 0.675
N-methyl, N-carboxymethyl-3,4-dicarboxybenzylamine
0.22
Periodate
-
-
140
perrhenate
-
-
-
17
phosphonate
-
-
11
tellurate
-
-
26
tungstate
-
-
20
z-olefin
-
-
-
additional information
additional information
-
inhibition kinetics at 21°C and pH 7.5
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.306
petrosamine B
Helicobacter pylori
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
-
K12 6201 strain, enriched media plus diaminopimelic acid
0.0029
-
of the recombinant in the crude extracts of Escherichia coli
0.01
-
grown in minimal medium or in minimal medium plus lysine, methionine and threonine
0.012
-
grown in minimal medium plus isoleucine
0.013
-
grown in minimal medium plus lysine or in minimal medium plus threonine
0.015
-
grown in minimal medium plus leucine or in minimal medium plus methionine
0.02
-
grown in complex medium
0.04
-
assay system plus lysine or assays system plus lysine, threonine and methionine
0.043
-
no addition to the assay system or assay system plus methionine
0.048
-
assay system plus threonine
0.12
-
B AC70R1 strain, enriched media
0.215
-
gene asd1
0.373
partially purified recombinant enzyme
0.4
-
K12 23631 strain, minimal media plus N-formyllysine
0.94
-
B AC70R1 strain, minimal media
1.8
-
K12 6201/PoP126 strain, enriched media plus ampicillin
2.149
-
gene asd2; when expressed in Escherichia coli
2.7
-
B AC70R1/PoP12 strain, enriched media plus tetracycline
5.3
-
B AC70R1/PoP12 strain, minimal media plus tetracycline
145
-
K12 23631/pOP12, after three times purification of the enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 7.8
-
mutant
7.5
-
assay at
8 - 9
-
L-aspartate 4-semialdehyde + arsenate
8
-
L-aspartyl phosphate + NADPH
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
pH 6.5: about 45% of activity maximum, mutant, about 8% of activity maximum, wild type, pH 9.0: about 55% of activity maximum, mutant, about 30% of activity maximum, wild type
8.4 - 9.5
-
at pH 8.4 and pH 9.5: 50% of maximal activity, L-aspartate 4-semialdehyde + phosphate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
activity assay
25
-
assay at
28
-
above 28°C, wild type
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Candida albicans (strain SC5314 / ATCC MYA-2876)
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
2 * 36000, SDS-PAGE
38000
-
2 * 38000, SDS-PAGE
38070
-
the expressed and purified monomer protein construct includes the 17-amino-acid sequence RGSHHHHHHGSACELGT between the N-terminal Met and the second amino acid Gly of the native Asd sequence, the total length of this protein is 362 amino acids
39000
-
2 * 39000, SDS-PAGE
39530
-
4 * 39530, nucleotide sequence analysis of the HOM2 region
40000
-
2 * 40000
41000
-
4 * 41000, SDS-PAGE
44000
-
2 * 44000, SDS-PAGE
45000
-
x * 45000, SDS-PAGE
70000
-
gel filtration
75000
-
homodimer in solution, determined by gel filtration; recombinant His-tagged enzyme, gel filtration
77000
-
sucrose density gradient centrifugation
77500
-
ultracentrifugation
140000
-
gel filtration
156000
-
sedimentation equilibrium, meniscus depletion technique
160000
recombinant His-tagged enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
tetramer
additional information