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Literature summary for 1.2.1.104 extracted from
- Purificaton of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and resolution of its four component polypeptides (1980), Biochem. J., 189, 161-172 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| The enzyme complex is resolved into its constituent proteins by means of gel filtration on Sepharose CL-6B in the presence of 2 M-KI, followed by chromatography on hydroxyapatite in the presence of 8 M urea. These harsh conditions are necessary to cause suitable dissociation of the enzyme complex | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
30°C, pH not specified in the publication | Geobacillus stearothermophilus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | x * 57000, x * 54000, x * 42000 and x * 36000, respectively, SDS-PAGE. The empirical unit must be repeated at least 50 times to make up the assembled complex | Geobacillus stearothermophilus |
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