KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.117 | - |
pyruvate | pH 7.0, 30°C | [Bacillus] caldolyticus | |
1.27 | - |
2-oxoisovalerate | pH 7.0, 30°C | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of pyruvate dehydrogenase complex including pyruvate decarboxylase E1 | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoisovalerate + CoA + NAD+ | - |
[Bacillus] caldolyticus | isobutanoyl-CoA + CO2 + NADH | - |
? | |
pyruvate + CoA + NAD+ | - |
[Bacillus] caldolyticus | acetyl-CoA + CO2 + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life 30 min, pyruvate decarboxylase subunit E1 | [Bacillus] caldolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
[Bacillus] caldolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.7 | 10.2 | pyruvate decarboxylase subunit E1 | [Bacillus] caldolyticus |