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Literature summary for 1.17.99.6 extracted from

  • Payne, K.A.; Fisher, K.; Sjuts, H.; Dunstan, M.S.; Bellina, B.; Johannissen, L.; Barran, P.; Hay, S.; Rigby, S.E.; Leys, D.
    Epoxyqueuosine reductase structure suggests a mechanism for cobalamin-dependent tRNA modification (2015), J. Biol. Chem., 290, 27572-27581 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus megaterium DSM319 cells Streptococcus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.3 M sodium acetate, 0.1 M Tris-HCl, pH 7.5, 15% (w/v) PEG 4000 Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
queuosine34 in tRNA + acceptor + H2O Streptococcus thermophilus
-
epoxyqueuosine34 in tRNA + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus A0A0R4I997
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, and heparin column chromatography Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epoxyqueuosine34 in tRNA + reduced methyl viologen
-
Streptococcus thermophilus queuosine34 in tRNA + methyl viologen + H2O
-
?
queuosine34 in tRNA + acceptor + H2O
-
Streptococcus thermophilus epoxyqueuosine34 in tRNA + reduced acceptor
-
?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Streptococcus thermophilus
? x * 47019, electrospray ionization mass spectrometry Streptococcus thermophilus

Synonyms

Synonyms Comment Organism
QueG
-
Streptococcus thermophilus

Cofactor

Cofactor Comment Organism Structure
Cobalamin
-
Streptococcus thermophilus
[4Fe-4S]-center
-
Streptococcus thermophilus