Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.99.6 extracted from

  • Miles, Z.D.; Myers, W.K.; Kincannon, W.M.; Britt, R.D.; Bandarian, V.
    Biochemical and spectroscopic studies of epoxyqueuosine reductase a novel iron-sulfur cluster- and cobalamin-containing protein involved in the biosynthesis of queuosine (2015), Biochemistry, 54, 4927-4935 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
queuosine34 in tRNA + acceptor + H2O Bacillus subtilis
-
epoxyqueuosine34 in tRNA + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
StrepTrap column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epoxyqueuosine34 in tRNA + reduced methyl viologen
-
Bacillus subtilis queuosine34 in tRNA + methyl viologen + H2O
-
?
queuosine34 in tRNA + acceptor + H2O
-
Bacillus subtilis epoxyqueuosine34 in tRNA + reduced acceptor
-
?

Subunits

Subunits Comment Organism
? x * 48700, calculated from amino acid sequence Bacillus subtilis
? x * 50000. SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
QueG
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
Cobalamin
-
Bacillus subtilis
[4Fe-4S]-center two [4Fe-4S] clusters Bacillus subtilis