Literature summary for 1.17.1.9 extracted from

Oezguen, G.P.; Ordu, E.B.; Tuetuencue, H.E.; Yelboga, E.; Sessions, R.B.; Guel Karagueler, N.
Site saturation mutagenesis applications on Candida methylica formate dehydrogenase (2016), Scientifica, 2016, 4902450 .

Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	[Candida] methylica

Protein Variants

Protein Variants	Comment	Organism
D195S	the mutant with decreased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica
D195S/Q197T	the mutant with increased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica
D195S/Q197V	the mutant with decreased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica
D195S/Y196A	the mutant with increased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica
D195S/Y196L	the mutant with decreased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica
D195S/Y196S	the mutant with decreased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica
Q197V	the mutant with decreased activity with NAD+ compared to the wild type enzyme also shows activity with NADP+	[Candida] methylica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.13	-	NAD+	mutant enzyme D195S/Y196A, at pH 8.0 and 25°C	[Candida] methylica
0.22	-	NAD+	mutant enzyme D195S/Q197T, at pH 8.0 and 25°C	[Candida] methylica
0.62	-	NAD+	wild type enzyme, at pH 8.0 and 25°C	[Candida] methylica
0.7	-	NAD+	mutant enzyme D195S/Y196L, at pH 8.0 and 25°C	[Candida] methylica
1.1	-	NAD+	mutant enzyme D195S, at pH 8.0 and 25°C	[Candida] methylica
1.4	-	NADP+	mutant enzyme Q197V, at pH 8.0 and 25°C	[Candida] methylica
2	-	NADP+	mutant enzyme D195S/Y196L, at pH 8.0 and 25°C	[Candida] methylica
2.2	-	NADP+	mutant enzyme D195S/Q197V, at pH 8.0 and 25°C	[Candida] methylica
2.3	-	NAD+	mutant enzyme D195S/Y196S, at pH 8.0 and 25°C	[Candida] methylica
3.3	-	NAD+	mutant enzyme Q197V, at pH 8.0 and 25°C	[Candida] methylica
3.9	-	NAD+	mutant enzyme D195S/Q197V, at pH 8.0 and 25°C	[Candida] methylica
4.6	-	NADP+	mutant enzyme D195S/Q197T, at pH 8.0 and 25°C	[Candida] methylica
4.75	-	formate	wild type enzyme, at pH 8.0 and 25°C	[Candida] methylica
5	-	NADP+	mutant enzyme D195S, at pH 8.0 and 25°C	[Candida] methylica
8.2	-	NADP+	mutant enzyme D195S/Y196S, at pH 8.0 and 25°C	[Candida] methylica
8.5	-	NADP+	mutant enzyme D195S/Y196A, at pH 8.0 and 25°C	[Candida] methylica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
formate + NAD+	[Candida] methylica	-	CO2 + NADH + H+	-	?
formate + NADP+	[Candida] methylica	NADP+ is a substrate for mutant enzymes only	CO2 + NAPDH	-	?
additional information	[Candida] methylica	the wild type enzyme shows no activity with NADP+	?	-	?

Organism

Organism	UniProt	Comment	Textmining
[Candida] methylica	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	[Candida] methylica	CO2 + NADH + H+	-	?
formate + NADP+	NADP+ is a substrate for mutant enzymes only	[Candida] methylica	CO2 + NAPDH	-	?
additional information	the wild type enzyme shows no activity with NADP+	[Candida] methylica	?	-	?

Synonyms

Synonyms	Comment	Organism
FDH	-	[Candida] methylica
NAD+-dependent formate dehydrogenase	-	[Candida] methylica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	NADP+	mutant enzyme D195S/Q197V, at pH 8.0 and 25°C	[Candida] methylica
0.03	-	NADP+	mutant enzyme Q197V, at pH 8.0 and 25°C	[Candida] methylica
0.04	-	NADP+	mutant enzyme D195S, at pH 8.0 and 25°C	[Candida] methylica
0.1	-	NADP+	mutant enzyme D195S/Y196L, at pH 8.0 and 25°C	[Candida] methylica
0.16	-	NAD+	mutant enzyme D195S/Q197V, at pH 8.0 and 25°C	[Candida] methylica
0.16	-	NADP+	mutant enzyme D195S/Y196S, at pH 8.0 and 25°C	[Candida] methylica
0.17	-	NADP+	mutant enzyme D195S/Y196A, at pH 8.0 and 25°C	[Candida] methylica
0.2	-	NAD+	wild type enzyme, at pH 8.0 and 25°C	[Candida] methylica
0.2	-	NAD+	mutant enzyme D195S/Q197T, at pH 8.0 and 25°C	[Candida] methylica
0.2	-	NAD+	mutant enzyme D195S/Y196A, at pH 8.0 and 25°C	[Candida] methylica
0.2	-	NAD+	mutant enzyme D195S/Y196L, at pH 8.0 and 25°C	[Candida] methylica
0.2	-	NAD+	mutant enzyme D195S/Y196S, at pH 8.0 and 25°C	[Candida] methylica
0.22	-	NAD+	mutant enzyme D195S, at pH 8.0 and 25°C	[Candida] methylica
0.22	-	NAD+	mutant enzyme Q197V, at pH 8.0 and 25°C	[Candida] methylica
0.26	-	NADP+	mutant enzyme D195S/Q197T, at pH 8.0 and 25°C	[Candida] methylica
1.1	-	formate	wild type enzyme, at pH 8.0 and 25°C	[Candida] methylica

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	[Candida] methylica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.24	-	formate	wild type enzyme, at pH 8.0 and 25°C	[Candida] methylica
4.5	-	NADP+	mutant enzyme D195S/Q197V, at pH 8.0 and 25°C	[Candida] methylica
8	-	NADP+	mutant enzyme D195S, at pH 8.0 and 25°C	[Candida] methylica
18	-	NADP+	mutant enzyme Q197V, at pH 8.0 and 25°C	[Candida] methylica
19	-	NADP+	mutant enzyme D195S/Y196S, at pH 8.0 and 25°C	[Candida] methylica
20	-	NADP+	mutant enzyme D195S/Y196A, at pH 8.0 and 25°C	[Candida] methylica
40	-	NAD+	mutant enzyme D195S/Q197V, at pH 8.0 and 25°C	[Candida] methylica
50	-	NADP+	mutant enzyme D195S/Y196L, at pH 8.0 and 25°C	[Candida] methylica
56	-	NADP+	mutant enzyme D195S/Q197T, at pH 8.0 and 25°C	[Candida] methylica
60	-	NAD+	mutant enzyme Q197V, at pH 8.0 and 25°C	[Candida] methylica
90	-	NAD+	mutant enzyme D195S/Y196S, at pH 8.0 and 25°C	[Candida] methylica
220	-	NAD+	mutant enzyme D195S, at pH 8.0 and 25°C	[Candida] methylica
260	-	NAD+	mutant enzyme D195S/Y196L, at pH 8.0 and 25°C	[Candida] methylica
320	-	NAD+	wild type enzyme, at pH 8.0 and 25°C	[Candida] methylica
900	-	NAD+	mutant enzyme D195S/Q197T, at pH 8.0 and 25°C	[Candida] methylica
1460	-	NAD+	mutant enzyme D195S/Y196A, at pH 8.0 and 25°C	[Candida] methylica

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Release 2023.1 (January 2023)