Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADH | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
0.4 | - |
CO2 | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
1.2 | - |
NAD+ | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
7.2 | - |
formate | at pH 10.5 and 35°C | Thermothelomyces thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | G2QB71 | - |
- |
Thermothelomyces thermophilus ATCC 42464 | G2QB71 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Thermothelomyces thermophilus |
Storage Stability | Organism |
---|---|
-20°C, glycine buffer, pH 10.5, 30 days, 40% loss of activity | Thermothelomyces thermophilus |
-80°C, glycine buffer, pH 10.5, 30 days, 50% loss of activity | Thermothelomyces thermophilus |
25°C, glycine buffer, pH 10.5, 30 days, complete loss of activity | Thermothelomyces thermophilus |
4°C, glycine buffer, pH 10.5, 30 days, 40% loss of activity | Thermothelomyces thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADH | - |
Thermothelomyces thermophilus | formate + NAD+ | - |
r | |
CO2 + NADH | - |
Thermothelomyces thermophilus ATCC 42464 | formate + NAD+ | - |
r | |
formate + NAD+ | - |
Thermothelomyces thermophilus | CO2 + NADH + H+ | - |
r | |
formate + NAD+ | - |
Thermothelomyces thermophilus ATCC 42464 | CO2 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Thermothelomyces thermophilus |
NAD+-dependent formate dehydrogenase | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Thermothelomyces thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 49 | about 78% activity at 25°C, 100% activity at 35°C, about 90% activity at 45°C, about 67% activity at 47°C, and about 40% activity at 49°C. No activity at 65°C | Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 48 | melting temperature is 48°C. The enzyme remains stable for up to 30 min at 25°C | Thermothelomyces thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADH | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
0.1 | - |
CO2 | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
0.3 | - |
formate | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
0.76 | - |
NAD+ | at pH 10.5 and 35°C | Thermothelomyces thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | the enzyme displays a sharp pH optimum for the reaction with sodium bicarbonate at pH pH 5.5-6.0 | Thermothelomyces thermophilus |
10.5 | - |
the enzyme displays a pH optimum for the conversion of formate anion to carbon dioxide at pH pH 10.5 | Thermothelomyces thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermothelomyces thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermothelomyces thermophilus | isoelectric focusing | - |
6.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
formate | at pH 10.5 and 35°C | Thermothelomyces thermophilus | |
0.17 | - |
NADH | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
0.23 | - |
CO2 | at pH 6.0 and 35°C | Thermothelomyces thermophilus | |
0.63 | - |
NAD+ | at pH 10.5 and 35°C | Thermothelomyces thermophilus |