Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Moraxella sp. C-1 |
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. 101 |
Protein Variants | Comment | Organism |
---|---|---|
A198G | the mutant shows 1.8 fold increased activity compared to the wild type enzyme | Moraxella sp. C-1 |
A198G | the mutation reduces the KM value for NAD+ while the KM for CO2 remains practically unchanged, compared to the wild type enzyme | Pseudomonas sp. 101 |
A198G/D221S | the mutant has its coenzyme specificity changed from NAD+ to NADP+ compared to the wild type enzyme | Pseudomonas sp. 101 |
D221S | the mutant has its coenzyme specificity changed from NAD+ to NADP+ compared to the wild type enzyme | Pseudomonas sp. 101 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
NAD+ | mutant enzyme A198G, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
0.045 | - |
NAD+ | mutant enzyme A198G, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
0.06 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
0.08 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
0.19 | - |
NADP+ | mutant enzyme D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
0.54 | - |
NAD+ | mutant enzyme A198G/D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
0.71 | - |
NAD+ | mutant enzyme D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
6.5 | - |
CO2 | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
7.5 | - |
CO2 | mutant enzyme A198G, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
7.7 | - |
CO2 | wild type enzyme, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
8 | - |
CO2 | mutant enzyme A198G, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
32 | - |
CO2 | mutant enzyme D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | Moraxella sp. C-1 | - |
CO2 + NADH + H+ | - |
? | |
formate + NAD+ | Pseudomonas sp. 101 | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moraxella sp. C-1 | O08375 | - |
- |
Pseudomonas sp. 101 | P33160 | - |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose column chromatography and Sephadex G-25 gel filtration | Moraxella sp. C-1 |
phenyl Sepharose column chromatography and Sephadex G-25 gel filtration | Pseudomonas sp. 101 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Moraxella sp. C-1 | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Pseudomonas sp. 101 | CO2 + NADH + H+ | - |
? | |
formate + NADP+ | NADP+ is a substrate for mutant enzyme D221S only | Pseudomonas sp. 101 | CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Moraxella sp. C-1 |
FDH | - |
Pseudomonas sp. 101 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
NAD+ | mutant enzyme D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
7.3 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
7.3 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
7.3 | - |
NAD+ | mutant enzyme A198G, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
7.3 | - |
NAD+ | mutant enzyme A198G, pH and temperature not specified in the publication | Pseudomonas sp. 101 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Moraxella sp. C-1 | |
NAD+ | - |
Pseudomonas sp. 101 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.04 | - |
NAD+ | mutant enzyme D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
9.26 | - |
NAD+ | mutant enzyme A198G/D221S, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
91.3 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
122 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas sp. 101 | |
162 | - |
NAD+ | mutant enzyme A198G, pH and temperature not specified in the publication | Moraxella sp. C-1 | |
209 | - |
NAD+ | mutant enzyme A198G, pH and temperature not specified in the publication | Pseudomonas sp. 101 |