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Literature summary for 1.17.1.9 extracted from

  • Oezguen, G.; Karagueler, N.; Turunen, O.; Turner, N.; Binay, B.
    Characterization of a new acidic NAD+-dependent formate dehydrogenase from thermophilic fungus Chaetomium thermophilum (2015), J. Mol. Catal. B, 122, 212-217.
No PubMed abstract available
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.79
-
 NAD+ at pH 7.4 and 25°C Thermochaetoides thermophila
5.2
-
 formate at pH 7.4 and 25°C Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45600
-
-
 Thermochaetoides thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Thermochaetoides thermophila
-
 CO2 + NADH + H+
-
 ?
formate + NAD+ Thermochaetoides thermophila DSM 1495
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila G0SGU4
-
-
Thermochaetoides thermophila DSM 1495 G0SGU4
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Thermochaetoides thermophila

Storage Stability

Storage Stability Organism
-80°C, 20 mM acetate buffer pH 5.0, 30 days, 15% loss of activity Thermochaetoides thermophila
25°C, 20 mM acetate buffer pH 5.0, 30 days, 19% loss of activity Thermochaetoides thermophila
4°C, 20 mM acetate buffer pH 5.0, 30 days, 3% loss of activity Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Thermochaetoides thermophila CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Thermochaetoides thermophila DSM 1495 CO2 + NADH + H+
-
 ?
additional information no activity with NADP+ Thermochaetoides thermophila ?
-
 ?
additional information no activity with NADP+ Thermochaetoides thermophila DSM 1495 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40845, calculated from amino acid sequence Thermochaetoides thermophila
? x * 45600, SDS-PAGE Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
FDH
-
 Thermochaetoides thermophila
NAD+-dependent formate dehydrogenase
-
 Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 60 the enzyme exhibits good stability up to 55°C. The activity is lost between 55°C and 60°C. The temperature at which half of the activity is left at pH 5.0 is determined as 58°C Thermochaetoides thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.65
-
 NAD+ at pH 7.4 and 25°C Thermochaetoides thermophila
0.81
-
 formate at pH 7.4 and 25°C Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
4 11.5 the enzyme is highly active between pH 4.0 and 11.5. The relative activity decreases quite slowly above pH 5.0, and the enzyme shows considerable activity at pH 9.5–11.5 Thermochaetoides thermophila

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermochaetoides thermophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 formate at pH 7.4 and 25°C Thermochaetoides thermophila
0.36
-
 NAD+ at pH 7.4 and 25°C Thermochaetoides thermophila