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Literature summary for 1.17.1.9 extracted from

  • Baack, R.D.; Markwell, J.; Herman, P.L.; Osterman, J.C.
    Kinetic behavior of the Arabidopsis thaliana leaf formate dehydrogenase is thermally sensitive (2003), J. Plant Physiol., 160, 445-450.
    View publication on PubMed
Search for more literature for 1.17.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 formate pH 7.5, 30°C, heated DE-52-fraction Arabidopsis thaliana
0.011
-
 formate pH 7.5, 30°C, enzyme expressed in tobacco Arabidopsis thaliana
0.011
-
 formate pH 7.5, 30°C, enzyme from Sephadex G-15 desalted fraction Arabidopsis thaliana
0.011
-
 formate pH 7.5, 30°C, mitochondrial enzyme Arabidopsis thaliana
0.012
-
 formate pH 7.5, 30°C, enzyme from DE-52 chromatography Arabidopsis thaliana
0.035
-
 NAD+ pH 7.5, 30°C, heated DE-52-fraction Arabidopsis thaliana
0.075
-
 NAD+ pH 7.5, 30°C, enzyme from DE-52 chromatography Arabidopsis thaliana
0.075
-
 NAD+ pH 7.5, 30°C, enzyme from Sephadex G-15 desalted fraction Arabidopsis thaliana
0.076
-
 NAD+ pH 7.5, 30°C, mitochondrial enzyme Arabidopsis thaliana
0.078
-
 NAD+ pH 7.5, 30°C, enzyme expressed in tobacco Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Arabidopsis thaliana

Renatured (Commentary)

Renatured (Comment) Organism
heating converts the native enzyme to the molten globule state and cooling results in formation of a nonnative structure with altered kinetic properties Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0013
-
 pH 7.5, 30°C, enzyme from Sephadex G-15 desalted fraction Arabidopsis thaliana
0.043
-
 pH 7.5, 30°C, enzyme from DE-52 chromatography Arabidopsis thaliana
0.058
-
 pH 7.5, 30°C, mitochondrial enzyme Arabidopsis thaliana
0.094
-
 pH 7.5, 30°C, heated DE-52-fraction Arabidopsis thaliana
1.256
-
 pH 7.5, 30°C, enzyme expressed in tobacco Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Arabidopsis thaliana CO2 + NADH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana