Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.54 extracted from

  • Patel, I.; Kracher, D.; Ma, S.; Garajova, S.; Haon, M.; Faulds, C.B.; Berrin, J.G.; Ludwig, R.; Record, E.
    Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6 (2016), Biotechnol. Biofuels, 9, 108.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.54

Activating Compound

Activating Compound Comment Organism Structure
cellobiose dehydrogenase physiological electron donor Pestalotiopsis sp. NCi6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Pestalotiopsis sp. NCi6

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pestalotiopsis sp. NCi6
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ strictly conserved copper-binding site which consists of two histidines (one at N-terminal position) and one tyrosine Pestalotiopsis sp. NCi6

Organism

Organism UniProt Comment Textmining
Pestalotiopsis sp. NCi6 KR825269 mangrove-associated fungus
-
Pestalotiopsis sp. NCi6 KR825270 mangrove-associated fungus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoric acid swollen cellulose + ascorbate + O2
-
 Pestalotiopsis sp. NCi6 C4-dehydro-cellooligosaccharide + dehydroascorbate + 2 H2O The chain lengths of the cellooligosaccharides ranges from 2 to 5 ?
phosphoric acid swollen cellulose + ascorbate + O2
-
 Pestalotiopsis sp. NCi6 C4-dehydro-cellooligosaccharide-C1-lactone + dehydroascorbate + H2O the initially formed lactone at the reducing end of the produced cellooligosaccharides is hydrolyzed spontanously to the aldonic acid. The chain lengths of the cellooligosaccharides ranges from 2 to 5 ?
phosphoric acid swollen cellulose + ascorbate + O2
-
 Pestalotiopsis sp. NCi6 cellooligosaccharide-C1-lactone + dehydroascorbate + H2O the initially formed lactone at the reducing end of the produced cellooligosaccharides is hydrolyzed spontanously to the aldonic acid. The chain lengths of the cellooligosaccharides ranges from 2 to 5 ?

Synonyms

Synonyms Comment Organism
PsLPMOA
-
 Pestalotiopsis sp. NCi6
PsLPMOB
-
 Pestalotiopsis sp. NCi6

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 100% activity after 150 min and approximately 80% after 1000 min of incubation Pestalotiopsis sp. NCi6
60
-
 considerably stable Pestalotiopsis sp. NCi6
60
-
 fast degradation Pestalotiopsis sp. NCi6

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pestalotiopsis sp. NCi6

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 no activity below pH 4.5 Pestalotiopsis sp. NCi6