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Literature summary for 1.14.99.53 extracted from

  • Loose, J.; Forsberg, Z.; Fraaije, M.; Eijsink, V.; Vaaje-Kolstad, G.
    A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase (2014), FEBS Lett., 588, 3435-3440.
    View publication on PubMed
Search for more literature for 1.14.99.53

Application

Application Comment Organism
analysis method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity Vibrio cholerae serotype O1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ saturation of enzyme with cu2+ prior to assay Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chitin + ascorbate + O2
-
 Vibrio cholerae serotype O1 C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
GbpA virulence factor with no obvious role in biomass processing Vibrio cholerae serotype O1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Vibrio cholerae serotype O1