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Literature summary for 1.14.20.4 extracted from

  • Nakajima, J.I.; Tanaka, Y.; Yamazaki, M.; Saito, K.
    Reaction mechanism from leucoanthocyanidin to anthocyanidin 3-glucoside, a key reaction for coloring in anthocyanin biosynthesis (2001), J. Biol. Chem., 276, 25797-25803.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate
-
 Zea mays
2-oxoglutarate
-
 Petunia x hybrida
2-oxoglutarate
-
 Perilla frutescens
ascorbate
-
 Zea mays
ascorbate
-
 Petunia x hybrida

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Zea mays
overexpression in Escherichia coli Petunia x hybrida
overexpression in Escherichia coli Perilla frutescens
overexpression in Escherichia coli Antirrhinum majus
overexpression in Escherichia coli Torenia fournieri

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Zea mays
Fe2+ dependent on Petunia x hybrida

Organism

Organism UniProt Comment Textmining
Antirrhinum majus
-
 snapdragon
-
Perilla frutescens
-
-
-
Petunia x hybrida
-
-
-
Torenia fournieri
-
-
-
Zea mays
-
 maize
-

Purification (Commentary)

Purification (Comment) Organism
of recombinant enzyme Zea mays
of recombinant enzyme Petunia x hybrida
of recombinant enzyme Antirrhinum majus
of recombinant enzyme Torenia fournieri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leucocyanidin + 2-oxoglutarate + O2 using (2R,3S,4R)-leucocyanidin or (2R,3S,4R)-leucopelargonidin as substrates Zea mays cis-dihydroquercetin + trans-dihydroquercetin + succinate + CO2
-
 ir
leucocyanidin + 2-oxoglutarate + O2 using (2R,3S,4R)-leucocyanidin or (2R,3S,4R)-leucopelargonidin as substrates Petunia x hybrida cis-dihydroquercetin + trans-dihydroquercetin + succinate + CO2
-
 ir
leucocyanidin + 2-oxoglutarate + O2 using (2R,3S,4R)-leucocyanidin or (2R,3S,4R)-leucopelargonidin as substrates Perilla frutescens cis-dihydroquercetin + trans-dihydroquercetin + succinate + CO2
-
 ir
leucocyanidin + 2-oxoglutarate + O2 using (2R,3S,4R)-leucocyanidin or (2R,3S,4R)-leucopelargonidin as substrates Antirrhinum majus cis-dihydroquercetin + trans-dihydroquercetin + succinate + CO2
-
 ir
leucocyanidin + 2-oxoglutarate + O2 using (2R,3S,4R)-leucocyanidin or (2R,3S,4R)-leucopelargonidin as substrates Torenia fournieri cis-dihydroquercetin + trans-dihydroquercetin + succinate + CO2
-
 ir