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Literature summary for 1.14.19.41 extracted from
- Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato (2006), Plant Cell, 18, 1008-1022.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a baculovirus/Sf9 insect cell system | Arabidopsis thaliana |
| expressed in a baculovirus/Sf9 insect cell system | Solanum lycopersicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
beta-sitosterol | at pH 7.3 and 30°C | Arabidopsis thaliana |  |
| 0.0037 | - |
beta-sitosterol | at pH 7.3 and 30°C | Solanum lycopersicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Arabidopsis thaliana | - |
- |
| microsome | - |
Solanum lycopersicum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Arabidopsis thaliana |
| 55000 | - |
x * 55000, SDS-PAGE | Solanum lycopersicum |
| 55723 | - |
x * 55723, calculated from amino acid sequence | Arabidopsis thaliana |
| 57511 | - |
x * 57511, calculated from amino acid sequence | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Columbia | - |
| Solanum lycopersicum | Q1T7C2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 24-epi-campesterol + NADPH + H+ + O2 | - |
Arabidopsis thaliana | brassicasterol + NADP+ + H2O | - |
? | |
| beta-sitosterol + NADPH + H+ + O2 | - |
Arabidopsis thaliana | stigmasterol + NADP+ + H2O | - |
? | |
| beta-sitosterol + NADPH + H+ + O2 | - |
Solanum lycopersicum | stigmasterol + NADP+ + H2O | - |
? | |
| additional information | no activity with campesterol | Arabidopsis thaliana | ? | - |
? | |
| additional information | no activity with campesterol | Solanum lycopersicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 55000, SDS-PAGE | Solanum lycopersicum |
| ? | x * 55723, calculated from amino acid sequence | Arabidopsis thaliana |
| ? | x * 57511, calculated from amino acid sequence | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP710A | - |
Arabidopsis thaliana |
| CYP710A11 | - |
Solanum lycopersicum |
| sterol C-22 desaturase | - |
Arabidopsis thaliana |
| sterol C-22 desaturase | - |
Solanum lycopersicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
beta-sitosterol | at pH 7.3 and 30°C | Arabidopsis thaliana | |
| 0.17 | - |
beta-sitosterol | at pH 7.3 and 30°C | Solanum lycopersicum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Arabidopsis thaliana | |
| NADPH | - |
Solanum lycopersicum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
beta-sitosterol | at pH 7.3 and 30°C | Arabidopsis thaliana | |
| 46.7 | - |
beta-sitosterol | at pH 7.3 and 30°C | Solanum lycopersicum | |
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