Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphorylation of TPH2 at Ser19 within the regulatory domain of the enzyme by calmodulin-dependent protein kinase II activates the enzyme | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of GST-tagged TPH2 in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S19A | site-directed mutagenesis, the mutant is not phpsphorylated by calmodulin-dependent protein kinase II and not activates, but basal activity and catalytic function is unaltered | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin + O2 | Homo sapiens | initial and rate-limiting enzyme in the biosynthesis of serotonin | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes TPH1 and TPPH2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of TPH2 at Ser19 by calmodulin-dependent protein kinase II activates the enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged TPH2 from Escherichia coli by glutathione affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | TPH1 is found in the periphery and pineal gland whereas TPH2 is expressed specifically in the central nervous system | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + 6-methyl-tetrahydropterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 6-methyl-4a-hydroxytetrahydropterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | initial and rate-limiting enzyme in the biosynthesis of serotonin | Homo sapiens | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPH2 | - |
Homo sapiens |
tryptophan hydroxylase 2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |