KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
L-tryptophan | enzyme from crude mitochondrial fraction | Cavia porcellus | |
0.006 | - |
2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine | soluble enzyme from brain | Cavia porcellus | |
0.3 | - |
L-tryptophan | soluble enzyme from brain | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | - |
Cavia porcellus | - |
- |
particle-bound | - |
Bos taurus | - |
- |
soluble | - |
Cavia porcellus | - |
- |
soluble | - |
Bos taurus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydropteridine + O2 | Cavia porcellus | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | Bos taurus | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cavia porcellus |
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | serotonergic neurons | Cavia porcellus | - |
brain | serotonergic neurons | Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, 2 months, 40% loss of activity | Cavia porcellus |
-20°C, 2 months, 40% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine + O2 | - |
Cavia porcellus | ? | - |
r | |
L-tryptophan + tetrahydropteridine + O2 | - |
Cavia porcellus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | - |
Bos taurus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | Cavia porcellus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | enzyme catalyzes the rate-limiting step in biosynthesis of putative neurotransmitter 5-hydroxytryptamine, serotonin | Bos taurus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | soluble enzyme from brain | Cavia porcellus |