Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | or other sulfhydryl reagents like 2-mercaptoethanol and L-cysteine are essential for activation, 5fold increase in activity | Mus musculus | |
L-cysteine | - |
Mus musculus |
General Stability | Organism |
---|---|
EDTA: 0.05 mM stabilizes | Mus musculus |
ethylene glycol, 50%, stabilizes | Mus musculus |
NaCl: 1 M, in addition to other stabilizing agents brings a great improvement in stabilization | Mus musculus |
Tween 20: 0.06% stabilizes | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Mus musculus | |
2,2'-dipyridyl | - |
Mus musculus | |
4-Fluorotryptophan | - |
Mus musculus | |
5-Fluorotryptophan | - |
Mus musculus | |
6-Fluorotryptophan | - |
Mus musculus | |
8-hydroxyquinoline | - |
Mus musculus | |
L-p-Chlorophenylalanine | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
L-tryptophan | cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine | Mus musculus | |
0.055 | - |
2-amino-4-hydroxy-6-methyltetrahydropteridine | substrate L-tryptophan | Mus musculus | |
0.1 | - |
L-phenylalanine | cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 5fold increase in activity | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
4 * 53000, enzyme from mastocytoma cells, SDS-PAGE | Mus musculus |
270000 | - |
enzyme from mastocytoma cells, gel filtration | Mus musculus |
280000 | - |
enzyme from mastocytoma cells, gradient PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydropteridine + O2 | Mus musculus | believed to be the rate-limeting enzyme in the biosynthesis of serotonin in the central nervous system | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine-agarose | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mastocytoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.28 | - |
purified from mastocytoma cells | Mus musculus |
Storage Stability | Organism |
---|---|
-80°C, 1 month, 30% loss of activity | Mus musculus |
4°C, 0.06% Tween 20, 0.05 mM EDTA, 50% ethylene glycol, 1 M NaCl, 5 days, no loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydropteridine + O2 | at a comparable rate of L-tryptophan hydroxylation | Mus musculus | tyrosine + dihydropteridine + H2O | - |
? | |
L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2 | - |
Mus musculus | ? | - |
r | |
L-tryptophan + tetrahydropteridine + O2 | cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine | Mus musculus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | believed to be the rate-limeting enzyme in the biosynthesis of serotonin in the central nervous system | Mus musculus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tyrosine + tetrahydropteridine + O2 | 1% of activity with L-tryptophan | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 53000, enzyme from mastocytoma cells, SDS-PAGE | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
complete loss of activity in absence of stabilizing agents after 20 h | Mus musculus |