BRENDA - Enzyme Database
show all sequences of 1.14.15.30

Multiplicity of 3-ketosteroid-9alpha-hydroxylase enzymes in Rhodococcus rhodochrous DSM43269 for specific degradation of different classes of steroids

Petrusma, M.; Hessels, G.; Dijkhuizen, L.; van der Geize, R.; J. Bacteriol. 193, 3931-3940 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA1, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3); gene kshA5, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. kshA5 supports growth on any of the different classes of steroids tested, consistent with its broad expression induction pattern. Recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3); gene kshB, recombinant expression of kshB in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Engineering
Amino acid exchange
Commentary
Organism
additional information
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol is severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked; a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Rhodococcus rhodochrous
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
additional information
Rhodococcus rhodochrous DSM 43269
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus rhodochrous
B6V6V5
isozyme KshA4
-
Rhodococcus rhodochrous
F1CMX0
gene kshA1 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA1
-
Rhodococcus rhodochrous
F1CMX3
gene kshB, encoding the ferredoxin reductase
-
Rhodococcus rhodochrous
F1CMX6
isozyme KshA2
-
Rhodococcus rhodochrous
F1CMX8
isozyme KshA3
-
Rhodococcus rhodochrous
F1CMY8
gene kshA5 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA5
-
Rhodococcus rhodochrous DSM 43269
B6V6V5
isozyme KshA4
-
Rhodococcus rhodochrous DSM 43269
F1CMX0
gene kshA1 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA1
-
Rhodococcus rhodochrous DSM 43269
F1CMX3
gene kshB, encoding the ferredoxin reductase
-
Rhodococcus rhodochrous DSM 43269
F1CMX6
isozyme KshA2
-
Rhodococcus rhodochrous DSM 43269
F1CMX8
isozyme KshA3
-
Rhodococcus rhodochrous DSM 43269
F1CMY8
gene kshA5 encodes an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase; isozyme KshA5
-
Purification (Commentary)
Commentary
Organism
recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3); recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3); recombinant KshB from Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
cell growth on different steroids as a sole carbon and energy source with different isozymes, overview; cell growth on different steroids as a sole carbon and energy source with different isozymes, overview; cell growth on different steroids as a sole carbon and energy source with different isozymes, overview
Rhodococcus rhodochrous
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 43% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 127% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 94% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 101% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 113% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA1 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA3 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA4 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA5 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 13% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 26% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 112% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 99% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 67% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-5alpha-androstane-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 9% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 64% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 19% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 58% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 288% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 194% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 89% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 49% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at; assay at; assay at
Rhodococcus rhodochrous
Cofactor
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
-
Rhodococcus rhodochrous
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells
Rhodococcus rhodochrous
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA1, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
expressed in Escherichia coli BL21(DE3) and C41(DE3) cells; gene kshA5, encoding an isoform of the oxygenase component of 3-ketosteroid 9alpha-hydroxylase, phylogenetic analysis and tree. Transcriptional analysis and functional complementation of kshA null mutant strain RG32 by genes kshA1-55, overview. kshA5 supports growth on any of the different classes of steroids tested, consistent with its broad expression induction pattern. Recombinant expression of kshA in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
gene kshB, recombinant expression of kshB in Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
-
Rhodococcus rhodochrous
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol is severely impaired, but not completely blocked
Rhodococcus rhodochrous
additional information
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Rhodococcus rhodochrous
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
additional information
Rhodococcus rhodochrous DSM 43269
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodococcus rhodochrous
; recombinant KshA from Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
recombinant KshB from Escherichia coli strains Bl21(DE3) and C41(DE3)
Rhodococcus rhodochrous
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
cell growth on different steroids as a sole carbon and energy source with different isozymes, overview
Rhodococcus rhodochrous
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 68% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 28% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-(5alpha)-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-5alpha-androst-1-en-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
11beta-hydrocortisone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-11beta-hydrocortisone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 78% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 24% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 10% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
19-nor-4-androstene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 107% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-19-nor-4-androstene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 500% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholest-4-en-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 124% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholest-4-en-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 43% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 22% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 176% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxy-23,24-bisnorcholesta-1,4-dien-22-oic acid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
3-oxosteroid + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxysteroid + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 127% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 94% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 101% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 113% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-4-androstene-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA1 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA3 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA4 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-androstene-3,17-dione + O2 + NADH + H+
isozyme KshA5 shows 100% activity
717735
Rhodococcus rhodochrous
? + H2O + NAD+
-
-
-
?
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 13% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 23% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-cholestene-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 26% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 423% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 112% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 99% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
4-pregnene-3,20-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 67% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-4-pregnene-3,20-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-17beta-ol-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
i.e. stanolon, isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha,17beta-dihydroxy-5alpha-androstane-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 9% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 64% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5alpha-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 80% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5alpha-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 19% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 58% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5beta-androstane-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 93% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxy-5beta-androstane-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA1 shows 288% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA3 shows 194% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA4 shows 89% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
isozyme KshA5 shows 49% activity compared to 4-androstene-3,17-dione
717735
Rhodococcus rhodochrous
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
717735
Rhodococcus rhodochrous DSM 43269
9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous
?
-
-
-
-
additional information
no activity with 4-cholestene-3-one, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
all KSH enzymes with KshADSM43269 isozymes are C-9alpha-hydroxylases acting on a wide range of 3-oxosteroids, but not on 3-hydroxysteroids
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
no activity with 5alpha-androstane-3,17-dione, 5beta-androstane-3,17-dione, 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, 3beta-hydroxy-5alpha-androstane-17-one, 5alpha-androstane-17beta-ol-3-one (stanolon) and 11beta-hydrocortisone
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
additional information
isozyme KshA5 is the most versatile isoenzyme, with the broadest substrate range but without a clear substrate preference. It shows no activity with 5-cholestene-3beta-ol (cholesterol), 5alpha-androstane-17-one, 3alpha-hydroxy-5alpha-pregnane-20-one, and 3beta-hydroxy-5alpha-androstane-17-one
717735
Rhodococcus rhodochrous DSM 43269
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Rhodococcus rhodochrous
Expression
Organism
Commentary
Expression
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, ksh1 is specifically induced by cholic acid; in wild type Rhodococcus rhodochrous strain DSM43269, kshA2 is specifically induced by 4-androstene-3,17-dione, progesterone, and cholic acid; in wild type Rhodococcus rhodochrous strain DSM43269, kshA3 is specifically induced by 4-androstene-3,17-dione; in wild type Rhodococcus rhodochrous strain DSM43269, kshA4 is specifically induced by 4-androstene-3,17-dione, cholic acid, and cholesterol; in wild type Rhodococcus rhodochrous strain DSM43269, kshA5 is specifically induced by 4-androstene-3,17-dione, progesterone, cholic acid, and cholesterol
up
General Information
General Information
Commentary
Organism
metabolism
3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism
Rhodococcus rhodochrous
additional information
KshA and KshB together constitute the KSH enzyme; KshA and KshB together constitute the KSH enzyme; KshA and KshB together constitute the KSH enzyme
Rhodococcus rhodochrous
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism; 3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA1 is dedicated to cholic acid catabolism. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation; 3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA5 appears to be the most versatile enzyme, with the broadest substrate range but without a clear substrate preference. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM 43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation
Rhodococcus rhodochrous
General Information (protein specific)
General Information
Commentary
Organism
metabolism
3-ketosteroid-9alpha-hydroxylase is a key enzyme in steroid catabolism
Rhodococcus rhodochrous
additional information
KshA and KshB together constitute the KSH enzyme
Rhodococcus rhodochrous
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA1 is dedicated to cholic acid catabolism. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation
Rhodococcus rhodochrous
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism. Isozyme KshA5 appears to be the most versatile enzyme, with the broadest substrate range but without a clear substrate preference. Presence of multiple kshA genes in the Rhodococcus rhodochrous DSM 43269 genome, each displaying unique steroid induction patterns and substrate ranges, appears to facilitate a dynamic and fine-tuned steroid catabolism, with C-9 alpha-hydroxylation occurring at different levels during microbial steroid degradation
Rhodococcus rhodochrous
physiological function
3-ketosteroid 9alpha-hydroxylase is a key enzyme in steroid catabolism
Rhodococcus rhodochrous
Expression (protein specific)
Organism
Commentary
Expression
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, ksh1 is specifically induced by cholic acid
up
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA5 is specifically induced by 4-androstene-3,17-dione, progesterone, cholic acid, and cholesterol
up
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA2 is specifically induced by 4-androstene-3,17-dione, progesterone, and cholic acid
up
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA3 is specifically induced by 4-androstene-3,17-dione
up
Rhodococcus rhodochrous
in wild type Rhodococcus rhodochrous strain DSM43269, kshA4 is specifically induced by 4-androstene-3,17-dione, cholic acid, and cholesterol
up
Other publictions for EC 1.14.15.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745648
Guevara
Functional characterization o ...
Rhodococcus ruber, Rhodococcus ruber Chol-4
J. Steroid Biochem. Mol. Biol.
172
176-187
2017
-
-
1
-
1
-
-
-
-
-
-
4
-
6
-
-
-
-
-
-
-
-
22
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
22
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
746503
Zhang
Efficient 9alpha-hydroxy-4-an ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
SpringerPlus
5
1207
2016
-
-
1
-
1
-
5
-
-
-
-
8
-
2
-
-
1
-
-
-
1
1
10
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
5
-
-
-
-
-
8
-
-
-
1
-
-
1
1
10
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
743221
Yao
Characterization and engineer ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum ATCC 25795, Mycolicibacterium neoaurum NwIB-01
Metab. Eng.
24
181-191
2014
-
-
1
-
1
-
-
-
-
1
-
3
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
744058
Petrusma
3-Ketosteroid 9alpha-hydroxyl ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2 155, Rhodococcus erythropolis, Rhodococcus erythropolis SQ1, Rhodococcus jostii, Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
Antonie van Leeuwenhoek
106
157-172
2014
-
2
2
-
1
-
-
-
-
5
-
22
-
15
-
-
-
5
-
-
-
-
28
5
-
-
-
-
-
-
-
10
-
-
-
-
2
4
14
-
1
-
-
-
-
-
-
7
-
22
-
-
-
-
-
-
-
-
28
7
-
-
-
-
-
-
-
-
-
20
28
-
-
-
745308
Penfield
Substrate specificities and c ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM43269, Rhodococcus rhodochrous DSM 43269
J. Biol. Chem.
289
25523-25536
2014
-
-
1
2
-
-
4
19
-
-
-
8
-
18
-
-
1
2
-
-
-
-
54
2
2
-
-
19
2
-
-
-
3
-
-
-
-
2
-
3
-
-
-
6
3
20
-
-
-
8
-
-
-
2
-
-
-
-
54
3
3
-
-
19
3
-
-
-
-
4
6
-
17
17
745698
Yeh
Deletion of the gene encoding ...
Rhodococcus hoagii 103S, Rhodococcus hoagii
Microb. Cell Fact.
13
130
2014
-
-
1
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
717737
Petrusma
Structural features in the Ksh ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
194
115-121
2012
-
-
1
-
8
-
-
2
-
1
-
3
-
13
-
-
1
-
-
-
-
-
74
-
-
-
-
-
1
-
-
2
-
-
-
-
-
3
4
-
16
-
-
-
-
4
-
2
-
3
-
-
-
3
-
-
-
-
74
-
-
-
-
-
2
-
-
-
-
1
2
-
-
-
717735
Petrusma
Multiplicity of 3-ketosteroid- ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous DSM 43269
J. Bacteriol.
193
3931-3940
2011
-
-
1
-
1
-
-
-
-
-
-
4
-
19
-
-
1
-
-
1
-
-
75
-
-
-
-
-
1
-
-
1
-
-
-
-
-
6
5
-
5
-
-
-
-
-
-
-
-
4
-
-
-
6
-
3
-
-
75
-
-
-
-
-
3
-
-
-
1
3
11
5
-
-
717854
Capyk
Activity of 3-ketosteroid 9alp ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
286
40717-40724
2011
-
-
-
-
-
-
-
13
-
-
-
2
-
9
-
-
-
-
-
-
-
-
30
1
1
-
-
11
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
21
-
-
-
2
-
-
-
-
-
-
-
-
30
2
2
-
-
19
2
-
-
-
-
2
3
-
13
19
713784
Wei
A new steroid-transforming str ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
716250
Hu
3-Ketosteroid 9alpha-hydroxyla ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
75
107-121
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
160
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
717500
Fan
Cloning, heterologous expressi ...
Mycobacterium sp., Mycobacterium sp. NwIB-01
Chin. J. Biotechnol.
25
2014-2021
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
8
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717784
Capyk
Characterization of 3-ketoster ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
284
9937-9946
2009
-
-
1
1
-
-
-
3
-
-
-
-
-
6
-
-
1
-
-
-
-
-
4
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
717119
Van Der Geize
Characterization of a second R ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Appl. Environ. Microbiol.
74
7197-7203
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
1
2
3
1
-
-
717118
Andor
Generation of useful insertion ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Appl. Environ. Microbiol.
72
6554-6559
2006
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718125
Van Der Geize
Molecular and functional chara ...
Rhodococcus erythropolis, Rhodococcus erythropolis SQ1
Mol. Microbiol.
45
1007-1018
2002
-
-
-
-
1
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-