BRENDA - Enzyme Database
show all sequences of 1.14.15.25

p-Cymene catabolic pathway in Pseudomonas putida F1 cloning and characterization of DNA encoding conversion of p-cymene to p-cumate

Eaton, R.W.; J. Bacteriol. 179, 3171-3180 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
O33456 and O33457
O33456 i.e. hydroxylase subunit CymAa, O33457 i.e. reductase subunit CymAb, cf. EC 1.8.1.3
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme can attack benzylic and toluic alcohols as well as toluene and xylenes, probably with the formation of gem-diol intermediates which spontaneously dehydrate to yield the corresponding aldehydes
745198
Pseudomonas putida
?
-
-
-
-
p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
745198
Pseudomonas putida
4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 43117, hydrolase subunit CymAa, x * 38295, reductase subunit CymAb, calculated
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
reductase subunit CymAb
Pseudomonas putida
[2Fe-2S]-center
reductase subunit CymAb
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
reductase subunit CymAb
Pseudomonas putida
[2Fe-2S]-center
reductase subunit CymAb
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme can attack benzylic and toluic alcohols as well as toluene and xylenes, probably with the formation of gem-diol intermediates which spontaneously dehydrate to yield the corresponding aldehydes
745198
Pseudomonas putida
?
-
-
-
-
p-cymene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
745198
Pseudomonas putida
4-isopropylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43117, hydrolase subunit CymAa, x * 38295, reductase subunit CymAb, calculated
Pseudomonas putida
Other publictions for EC 1.14.15.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746333
Dutta
-
Purification and properties o ...
Pseudomonas chlororaphis subsp. aureofaciens
Process Biochem.
47
1263-1267
2012
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
1
-
-
-
1
-
-
1
-
-
746417
Dutta
Cloning and characterization ...
Pseudomonas chlororaphis subsp. aureofaciens
Res. Microbiol.
161
876-882
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744134
Nishio
Biotransformations catalyzed ...
Pseudomonas putida
Appl. Microbiol. Biotechnol.
55
321-325
2001
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744208
Dutta
Reductase gene sequences and ...
Pseudomonas chlororaphis subsp. aureofaciens
Biochem. Biophys. Res. Commun.
233
502-506
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745198
Eaton
p-Cymene catabolic pathway in ...
Pseudomonas putida
J. Bacteriol.
179
3171-3180
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-