BRENDA - Enzyme Database
show all sequences of 1.14.15.14

Biochemical and structural characterization of CYP124: a methyl-branched lipid omega-hydroxylase from Mycobacterium tuberculosis

Johnston, J.B.; Kells, P.M.; Podust, L.M.; Ortiz de Montellano, P.R.; Proc. Natl. Acad. Sci. USA 106, 20687-20692 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, free enzyme using 1% (w/v) PEGMME 2000, 0.1 M Na cacodylate (pH 6.8), and 0.9 M succinic acid, and enzyme in complex with phytanic acid using 21% (w/v) PEG 4000, 0.1 M Bis-Tris (pH 5.5), and 0.2 M calcium acetate
Mycobacterium tuberculosis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.009
-
15-methylpalmitic acid
at pH 7.4 and 25°C
Mycobacterium tuberculosis
0.032
-
geranylgeraniol
at pH 7.4 and 25°C
Mycobacterium tuberculosis
0.036
-
farnesol
at pH 7.4 and 25°C
Mycobacterium tuberculosis
0.054
-
phytanic acid
at pH 7.4 and 25°C
Mycobacterium tuberculosis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48700
-
x * 48700, calculated from amino acid sequence
Mycobacterium tuberculosis
50000
-
x * 50000, SDS-PAGE
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
Mycobacterium tuberculosis
-
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
Mycobacterium tuberculosis H37Rv
-
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycobacterium tuberculosis
P9WPP3
-
-
Mycobacterium tuberculosis H37Rv
P9WPP3
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-NTA column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis H37Rv
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
best substrate
735155
Mycobacterium tuberculosis
?
-
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
best substrate
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
additional information
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
735155
Mycobacterium tuberculosis
?
-
-
-
-
additional information
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
-
palmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
the enzyme oxidizes the omega-1,omega-2, and omega-position of palmitic acid
735155
Mycobacterium tuberculosis
?
-
-
-
?
phytanic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 48700, calculated from amino acid sequence; x * 50000, SDS-PAGE
Mycobacterium tuberculosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, free enzyme using 1% (w/v) PEGMME 2000, 0.1 M Na cacodylate (pH 6.8), and 0.9 M succinic acid, and enzyme in complex with phytanic acid using 21% (w/v) PEG 4000, 0.1 M Bis-Tris (pH 5.5), and 0.2 M calcium acetate
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.009
-
15-methylpalmitic acid
at pH 7.4 and 25°C
Mycobacterium tuberculosis
0.032
-
geranylgeraniol
at pH 7.4 and 25°C
Mycobacterium tuberculosis
0.036
-
farnesol
at pH 7.4 and 25°C
Mycobacterium tuberculosis
0.054
-
phytanic acid
at pH 7.4 and 25°C
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48700
-
x * 48700, calculated from amino acid sequence
Mycobacterium tuberculosis
50000
-
x * 50000, SDS-PAGE
Mycobacterium tuberculosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
Mycobacterium tuberculosis
-
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
Mycobacterium tuberculosis H37Rv
-
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA column chromatography
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis H37Rv
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
best substrate
735155
Mycobacterium tuberculosis
?
-
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
best substrate
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
?
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
additional information
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
735155
Mycobacterium tuberculosis
?
-
-
-
-
additional information
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
735155
Mycobacterium tuberculosis H37Rv
?
-
-
-
-
palmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
the enzyme oxidizes the omega-1,omega-2, and omega-position of palmitic acid
735155
Mycobacterium tuberculosis
?
-
-
-
?
phytanic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
735155
Mycobacterium tuberculosis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 48700, calculated from amino acid sequence; x * 50000, SDS-PAGE
Mycobacterium tuberculosis
Other publictions for EC 1.14.15.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745645
Vasilevskaya
Identification of Mycobacteri ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Steroid Biochem. Mol. Biol.
169
202-209
2017
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5
-
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11
1
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1
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1
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11
1
-
-
-
-
-
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-
-
-
-
-
-
-
733555
Jensen
Photosystem I from plants as a ...
Mycobacterium tuberculosis
Biotechnol. Lett.
34
239-245
2012
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2
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1
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4
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5
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2
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1
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1
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2
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1
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5
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2
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-
735155
Johnston
Biochemical and structural cha ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
106
20687-20692
2009
-
-
1
1
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4
-
-
2
2
-
4
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1
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13
1
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1
-
1
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4
-
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2
2
-
-
-
1
-
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-
13
1
-
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