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Literature summary for 1.14.14.94 extracted from

  • Christmas, P.; Ursino, S.R.; Fox, J.W.; Soberman, R.J.
    Expression of the CYP4F3 gene. Tissue-specific splicing and alternative promoters generate high and low Km forms of leukotriene B4.omega-hydroxylase (1999), J. Biol. Chem., 274, 21191-21199.
    View publication on PubMed
Search for more literature for 1.14.14.94

Cloned(Commentary)

Cloned (Comment) Organism
chromosomal localization 19p Homo sapiens
CYP4F3 gene contains 14 exons and 13 introns Homo sapiens
expression in transfected COS-7 cells Homo sapiens
tissue-specific expression is regulated by alternative promoter usage and mutually exclusive exon splicing, exon 3 and 4 Homo sapiens
two isoforms of CYP4F3, CYP4F3A expressed in neutrophils and CYP4F3B expressed in fetal and adult liver Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 leukotriene B4 recombinant isoform CYP4F3A, expressed in COS-7 cells Homo sapiens
0.105
-
 leukotriene B4 recombinant isoform CYP4F3B, expressed in COS-7 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 recombinant isoforms CYP4F3A and CYP4F3B expressed in COS-7 cells, western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens inactivation of leukotriene B4, a mediator of inflammation (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver isoform CYP4F3B Homo sapiens
-
neutrophil isoform CYP4F3A Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 inactivation of leukotriene B4, a mediator of inflammation Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate Homo sapiens 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP4F3
-
 Homo sapiens
cytochrome P450 4F3
-
 Homo sapiens