Literature summary for 1.14.14.81 extracted from

Schwinn, K.; Miosic, S.; Davies, K.; Thill, J.; Gotame, T.P.; Stich, K.; Halbwirth, H.
The B-ring hydroxylation pattern of anthocyanins can be determined through activity of the flavonoid 3-hydroxylase on leucoanthocyanidins (2014), Planta, 240, 1003-1010 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.001	-	kaempferol	pH 7.0, 25°C	Billardiera heterophylla
0.001	-	naringenin	pH 7.0, 25°C	Billardiera heterophylla
0.0058	-	apigenin	pH 7.0, 25°C	Billardiera heterophylla
0.009	-	dihydrokaempferol	pH 7.0, 25°C	Billardiera heterophylla

Organism

Organism	UniProt	Comment	Textmining
Billardiera heterophylla	U3LXJ8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2	-	Billardiera heterophylla	? + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
apigenin + [reduced NADPH-hemoprotein reductase] + O2	-	Billardiera heterophylla	tricetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2	-	Billardiera heterophylla	dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O	-	?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2	-	Billardiera heterophylla	myricetin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2	-	Billardiera heterophylla	pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.6	-	naringenin	pH 7.0, 25°C	Billardiera heterophylla
13.4	-	apigenin	pH 7.0, 25°C	Billardiera heterophylla
17	-	kaempferol	pH 7.0, 25°C	Billardiera heterophylla
81.4	-	dihydrokaempferol	pH 7.0, 25°C	Billardiera heterophylla

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	-	Billardiera heterophylla

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
900	-	dihydrokaempferol	pH 7.0, 25°C	Billardiera heterophylla
1700	-	kaempferol	pH 7.0, 25°C	Billardiera heterophylla
2300	-	apigenin	pH 7.0, 25°C	Billardiera heterophylla
10000	-	naringenin	pH 7.0, 25°C	Billardiera heterophylla

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Release 2023.1 (January 2023)