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Literature summary for 1.14.14.3 extracted from

  • Phonbuppha, J.; Tinikul, R.; Chaiyen, P.
    Use of bacterial luciferase as a reporter gene in eukaryotic systems (2021), Methods Mol. Biol., 2274, 53-65 .
    View publication on PubMed

Application

Application Comment Organism
analysis a minimized cascade for Lux with greater ease of use, utilizes a chemoenzymatic reaction with biomimetic nicotinamide 1-benzyl-1,4-dihydronicotinamide in place of the flavin reductase reaction in the Lux system. The minimized cascade reaction can be applied to monitor bioluminescenceof the Lux reporter in eukaryotic cells effectively, and can achieve higher efficiencies than the system with flavin reductase Vibrio campbellii

Organism

Organism UniProt Comment Textmining
Vibrio campbellii
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a long-chain aldehyde + FMNH2 + O2 supply of FNH2 can be achieved by 1-benzyl-1,4-dihydronicotinamide instead of a flavin reductase system Vibrio campbellii a long-chain fatty acid + FMN + H2O + hv
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