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Literature summary for 1.14.14.178 extracted from

  • Fujita, S.; Ohnishi, T.; Watanabe, B.; Yokota, T.; Takatsuto, S.; Fujioka, S.; Yoshida, S.; Sakata, K.; Mizutani, M.
    Arabidopsis CYP90B1 catalyses the early C-22 hydroxylation of C27, C28 and C29 sterols (2006), Plant J., 45, 765-774.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 campesterol pH 7.25, 30°C Arabidopsis thaliana
0.003
-
 campestanol pH 7.25, 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 isoform CYP90B1, i.e. Dwf4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
campestanol + NADPH + H+ + O2 catalytic efficiency for campesterol is 325 times greater than for campestanol. 17% of the activity with cholesterol Arabidopsis thaliana 6-deoxocathasterone + NADP+ + H2O
-
 ?
campesterol + NADPH + H+ + O2 catalytic efficiency for campesterol is 325 times greater than for campestanol. 76.9% of the activity with cholesterol Arabidopsis thaliana 22alpha-hydroxycampesterol + NADP+ + H2O
-
 ?
cholestanol + NADPH + H+ + O2
-
 Arabidopsis thaliana 22-hydroxy-cholestanol + NADP+ + H2O 29% of the activity with cholesterol ?
cholesterol + NADPH + H+ + O2
-
 Arabidopsis thaliana 22-hydroxy-cholesterol + NADP+ + H2O
-
 ?
additional information substrates with a double bond at positions C5 and C6 are preferred substrates Arabidopsis thaliana ?
-
 ?
sitosterol + NADPH + H+ + O2 poor substrate. 10.7% of the activity with cholesterol Arabidopsis thaliana 22-hydroxy-sitosterol + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP90B1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00082
-
 campestanol pH 7.25, 30°C Arabidopsis thaliana
0.03
-
 campesterol pH 7.25, 30°C Arabidopsis thaliana