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Literature summary for 1.14.14.178 extracted from
- CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato (2006), Biosci. Biotechnol. Biochem., 70, 2071-2080.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in insect cell using a baculovirus expression system | Solanum lycopersicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Solanum lycopersicum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Solanum lycopersicum |
| 56000 | - |
x * 56000, SDS-PAGE | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Solanum lycopersicum | CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | A0ZS62 | cv. Momotarou | - |
| Solanum lycopersicum | A0ZS63 | cv. Momotarou | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Solanum lycopersicum | - |
| fruit | transcript in mature fruit and in immature fruit | Solanum lycopersicum | - |
| fruit | transcript in mature fruit, no transcript detected in immature fruit | Solanum lycopersicum | - |
| leaf | young | Solanum lycopersicum | - |
| seedling | - |
Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (24R)-ergost-4-en-3-one + NADPH + H+ + O2 | 17% of the activity with campesterol | Solanum lycopersicum | (22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O | - |
? | |
| (24R)-ergost-4-en-3-one + NADPH + H+ + O2 | 7.9% of the activity with campesterol | Solanum lycopersicum | (22S,24R)-22-hydroxy-ergost-4-en-3-one + NADP+ + H2O | - |
? | |
| campesterol + NADPH + H+ + O2 | best substrate | Solanum lycopersicum | (22S)-22-hydroxycampesterol + NADP+ + H2O | - |
? | |
| additional information | CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis | Solanum lycopersicum | ? | - |
? | |
| additional information | the enzyme catalyzes C-22 hydroxylation of (14R)-5alpha-ergostan-3-one and campestanol at a trace level | Solanum lycopersicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Solanum lycopersicum |
| ? | x * 55000, SDS-PAGE | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP724B2 | - |
Solanum lycopersicum |
| CYP90B3 | - |
Solanum lycopersicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Solanum lycopersicum | |
| NADPH | - |
Solanum lycopersicum | |
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Release 2023.1 (January 2023)
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