Cloned (Comment) | Organism |
---|---|
overexpression of PTDH-mFMO fusion protein in Escherichia coli | Methylophaga sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | preparation of self-sufficient monooxygenases by covalent coupling of mFMO with the soluble NADPHregenerating phosphite dehydrogenase, PTDH, from Pseudomonas stutzeri using a codon-optimized gene encoding a His-tagged and thermostable PTDH mutant as fusion partner. The bifunctional biocatalyst is able to use phosphite as a cheap and sacrificial substrate for recycling NADPH | Methylophaga sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Methylophaga sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Methylophaga sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophaga sp. | - |
- |
- |
Methylophaga sp. SK1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
one-step purification of the recombinant PTDH-mFMO from Escherichia coli by nickel affinity chromatography | Methylophaga sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-[4-(methylsulfanyl)phenyl]ethanone + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 21% R-enantiomer as product | Methylophaga sp. | (R,S)-1-[4-(methylsulfanyl)phenyl]ethanone S-oxide + NADP+ + H2O | - |
? | |
1-[4-(methylsulfanyl)phenyl]ethanone + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 21% R-enantiomer as product | Methylophaga sp. SK1 | (R,S)-1-[4-(methylsulfanyl)phenyl]ethanone S-oxide + NADP+ + H2O | - |
? | |
2-(methylsulfanyl)pyridine + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | 2-(methylsulfanyl)pyridine S-oxide + NADP+ + H2O | - |
? | |
2-(methylsulfanyl)pyridine + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. SK1 | 2-(methylsulfanyl)pyridine S-oxide + NADP+ + H2O | - |
? | |
2-(methylsulfanyl)thiophene + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | 2-(methylsulfanyl)thiophene S-oxide + NADP+ + H2O | - |
? | |
2-(methylsulfanyl)thiophene + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. SK1 | 2-(methylsulfanyl)thiophene S-oxide + NADP+ + H2O | - |
? | |
2-chlorophenyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 75% R-enantiomer as product | Methylophaga sp. | (R,S)-2-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
2-chlorophenyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 75% R-enantiomer as product | Methylophaga sp. SK1 | (R,S)-2-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
2-[(methylsulfanyl)methyl]furan + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | 2-[(methylsulfanyl)methyl]furan S-oxide + NADP+ + H2O | - |
? | |
3-chlorophenyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 15% R-enantiomer as product | Methylophaga sp. | (R,S)-3-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
4-(methylsulfanyl)benzonitrile + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 22% S-enantiomer as product | Methylophaga sp. | (R,S)-4-(methylsulfanyl)benzonitrile S-oxide + NADP+ + H2O | - |
? | |
4-(methylsulfanyl)phenol + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 81% S-enantiomer as product | Methylophaga sp. | (R,S)-4-(methylsulfanyl)phenol S-oxide + NADP+ + H2O | - |
? | |
4-chlorophenyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 95% S-enantiomer as product | Methylophaga sp. | (R,S)-4-chlorophenyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
benzyl ethyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | benzyl ethyl sulfide S-oxide + NADP+ + H2O | - |
? | |
benzyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | benzyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
butyl ethyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | butyl ethyl sulfide S-oxide + NADP+ + H2O | - |
? | |
butyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | butyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
cyclohexyl methyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | cyclohexyl methyl sulfide S-oxide + NADP+ + H2O | - |
? | |
ethyl phenyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | ethyl phenyl sulfoxide + NADP+ + H2O | - |
? | |
methyl 2-phenylethyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | methyl 2-phenylethyl sulfide S-oxide + NADP+ + H2O | - |
? | |
methyl 4-(methylsulfanyl)phenyl ether + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 70% S-enantiomer as product | Methylophaga sp. | (R,S)-methyl 4-(methylsulfanyl)phenyl ether S-oxide + NADP+ + H2O | - |
? | |
methyl 4-methylphenyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 92% S-enantiomer as product | Methylophaga sp. | (R,S)-methyl 4-methylphenyl sulfide S-oxide + NADP+ + H2O | - |
? | |
methyl 4-nitrophenyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 37% S-enantiomer as product | Methylophaga sp. | (R,S)-methyl 4-nitrophenyl sulfide S-oxide + NADP+ + H2O | - |
? | |
methyl phenyl sulfide + NADPH + H+ + O2 | sulfoxidation of the thioanisole derivative by recombinant PTDH-mFMO fusion protein. Enantiomeric reaction with 35% S-enantiomer as product | Methylophaga sp. | (R,S)-methyl phenyl sulfoxide + NADP+ + H2O | - |
? | |
additional information | substrate specificity of the recombinant PTDH-mFMO fusion enzyme, overview | Methylophaga sp. | ? | - |
? | |
additional information | substrate specificity of the recombinant PTDH-mFMO fusion enzyme, overview | Methylophaga sp. SK1 | ? | - |
? | |
phenyl propyl sulfide + NADPH + H+ + O2 | sulfoxidation by recombinant PTDH-mFMO fusion protein | Methylophaga sp. | phenyl propyl sulfide S-oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54000, SDS-PAGE | Methylophaga sp. |
Synonyms | Comment | Organism |
---|---|---|
FMO | - |
Methylophaga sp. |
mFMO | - |
Methylophaga sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
recombinant PTDH-mFMO | Methylophaga sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
purified recombinant PTDH-mFMO, half-life is 5 h | Methylophaga sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant PTDH-mFMO | Methylophaga sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Methylophaga sp. |