Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cholate | 29% activation at 0.5% concentration | Ovis aries | |
Emulgen 913 | 350% activation at 0.2%, 47% activation at 1.0% concentration | Ovis aries | |
Triton X-100 | 271% activation at 0.1%, 159% activation at 0.3% concentration | Ovis aries |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chlorpromazine | competitive inhibition of methimazole oxidation at high concentrations | Ovis aries | |
HgCl2 | IC50: 4.7 mM, liver microsomes | Ovis aries | |
imipramine | competitive inhibition of methimazole oxidation at high concentrations | Ovis aries | |
MgCl2 | IC50: 77.5 mM, liver microsomes | Ovis aries | |
n-octylamine | IC50: 4 mM, liver microsomes | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.118 | - |
Methimazole | pH 8.0, 37°C, liver microsomes | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | isozyme FMO3 | Ovis aries | - |
- |
nuclear envelope | - |
Ovis aries | 5635 | - |
smooth endoplasmic reticulum | - |
Ovis aries | 5790 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ovis aries | the enzyme is involved in detoxification, generally, metabolites produced by FMO-catalysed reactions are more hydrophilic and less toxic, and are easily excreted from the body | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
7-9-months-old Akkaraman sheep, isozyme FMO3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | isozyme FMO3 | Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0038 | - |
liver microsomes, substrate methimazole | Ovis aries |
0.0061 | - |
liver microsomes, substrate methimazole in presence of 0.1% Triton X-100 | Ovis aries |
0.0107 | - |
liver microsomes, substrate imipramine | Ovis aries |
0.0123 | - |
liver microsomes, substrate chlorpromazine | Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorpromazine + NADPH + O2 | liver microsomes | Ovis aries | ? | - |
? | |
imipramine + NADPH + O2 | liver microsomes | Ovis aries | ? | - |
? | |
methimazole + NADPH + O2 | liver microsomes | Ovis aries | ? | - |
? | |
additional information | the enzyme is involved in detoxification, generally, metabolites produced by FMO-catalysed reactions are more hydrophilic and less toxic, and are easily excreted from the body | Ovis aries | ? | - |
? | |
additional information | substrates are a wide range of nucleophilic nitrogen-, sulfur-, phosphorus-, and selenium heteroatom-containing chemicals, drugs, and agricultural agents | Ovis aries | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin monooxygenase | - |
Ovis aries |
FMO | - |
Ovis aries |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Ovis aries |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | temperature profile | Ovis aries |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
liver microsomes, enzyme inactivation after 20 days | Ovis aries |
37 | - |
liver microsomes, enzyme inactivation after 48 h | Ovis aries |
50 | - |
liver microsomes, enzyme inactivation after 1.5 h | Ovis aries |
65 | - |
liver microsomes, enzyme inactivation after 5 min | Ovis aries |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Ovis aries |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | pH profile | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme-bound | Ovis aries | |
NADPH | - |
Ovis aries |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
4 | - |
IC50: 4 mM, liver microsomes | Ovis aries | n-octylamine | |
4.7 | - |
IC50: 4.7 mM, liver microsomes | Ovis aries | HgCl2 | |
77.5 | - |
IC50: 77.5 mM, liver microsomes | Ovis aries | MgCl2 |