Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pentachlorphenol induces enzyme expression | Sphingobium chlorophenolicum |
Cloned (Comment) | Organism |
---|---|
gene pcpB, expression in Escherichia coli | Sphingobium chlorophenolicum |
gene pcpD, expression as C-terminally His12-tagged protein in Pseudomonas aeruginosa strain PAO1-Lac | Sphingobium chlorophenolicum |
Protein Variants | Comment | Organism |
---|---|---|
moe | construction of an allelic exchange mutant and a pcpD knockout mutant strains, the latter show reduced activity at high pentachlorophenol concentrations, not at low concentrations | Sphingobium chlorophenolicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrachlorobenzoquinone | inactivates the enzyme, feedback control | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sphingobium chlorophenolicum | enzyme expression in response to pentachlorophenol hydroxylase PcpB | ? | - |
? | |
pentachlorophenol + NADPH + H+ + O2 | Sphingobium chlorophenolicum | first step of pentachlorophenol degradation pathway | tetrachlorobenzoquinone + NADP+ + chloride + H2O | - |
ir | |
tetrachlorobenzoquinone + NADPH | Sphingobium chlorophenolicum | second step in pentachlorophenol degradation, physiological role in association with pentachloro monooxygenase, overview | tetrachlorohydroquinone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | - |
strain RA-2, strain ATCC 39723, gene pcpB | - |
Sphingobium chlorophenolicum | - |
strain RA-2, strain ATCC 39723, gene pcpD immediately downstream of gene pcpB | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli to homogeneity | Sphingobium chlorophenolicum |
recombinant enzyme from Pseudomonas aeruginosa to over 95% homogeneity by nickel affinity chromatography | Sphingobium chlorophenolicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | direct reduction by NADPH of the flavin at the active site without requirement of intermediate formation | Sphingobium chlorophenolicum | |
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | tetrachlorobenzoquinone reductase PcpD reaction mechanism | Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme expression in response to pentachlorophenol hydroxylase PcpB | Sphingobium chlorophenolicum | ? | - |
? | |
pentachlorophenol + NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | tetrachlorobenzoquinone + NADP+ + chloride + H2O | - |
ir | |
pentachlorophenol + NADPH + H+ + O2 | first step of pentachlorophenol degradation pathway | Sphingobium chlorophenolicum | tetrachlorobenzoquinone + NADP+ + chloride + H2O | - |
ir | |
tetrachlorobenzoquinone + NADPH | - |
Sphingobium chlorophenolicum | tetrachlorohydroquinone + NADP+ | - |
? | |
tetrachlorobenzoquinone + NADPH | second step in pentachlorophenol degradation, physiological role in association with pentachloro monooxygenase, overview | Sphingobium chlorophenolicum | tetrachlorohydroquinone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PCB hydroxylase | - |
Sphingobium chlorophenolicum |
PcpB | - |
Sphingobium chlorophenolicum |
PcpD | - |
Sphingobium chlorophenolicum |
pentachlorophenol hydroxylase | - |
Sphingobium chlorophenolicum |
pentachlorophenol monooxygenase | - |
Sphingobium chlorophenolicum |
tetrachlorobenzoquinone reductase | - |
Sphingobium chlorophenolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sphingobium chlorophenolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sphingobium chlorophenolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavin monooxygenase | Sphingobium chlorophenolicum | |
NADPH | - |
Sphingobium chlorophenolicum | |
NADPH | dependent on | Sphingobium chlorophenolicum |