Any feedback?
Information on EC 1.14.13.50 - pentachlorophenol monooxygenase
for references in articles please use BRENDA:EC1.14.13.50Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.14.13.50 pentachlorophenol monooxygenaseIUBMB Comments
A flavoprotein (FAD). The enzyme displaces a diverse range of substituents from the 4-position of polyhalogenated phenols but requires that a halogen substituent be present at the 2-position . If C-4 carries a halogen substituent, reaction 1 is catalysed; if C-4 is unsubstituted, reaction 2 is catalysed.
Specify your search results
Word Map
- 1.14.13.50
-
pcp-degrading
- flavobacterium
-
sphingobium
- chlorophenolicum
- tetrachlorohydroquinone
-
bioremediation
- tetrachlorobenzoquinone
-
dehalogenation
- environmental protection
- arthrobacter
- degradation
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
pcp hydroxylase, pcp-4-monooxygenase, pentachlorophenol-4-monooxygenase, pentachlorophenol 4-monooxygenase, pcp4mo, pentachlorophenol hydroxylase, pcp 4-monooxygenase, phe4mo, pcp-monooxygenase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
dechlorinase, pentachlorophenol
-
-
-
-
GM610_18120
oxygenase, pentachlorophenol 4-mono-
-
-
-
-
PCP 4-monooxygenase
PCP hydroxylase
PCP-4-monooxygenase
PcpB
pentachlorophenol 4-mono-oxygenase
-
-
-
-
pentachlorophenol 4-monooxygenase
pentachlorophenol dechlorinase
-
-
-
-
pentachlorophenol dehalogenase
-
-
-
-
pentachlorophenol hydroxylase
pentachlorophenol-4-monooxygenase
Phe4MO
PCP hydroxylase
-
-
-
-
pentachlorophenol 4-monooxygenase
-
-
-
-
pentachlorophenol hydroxylase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2,3,5,6-tetrachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachlorohydroquinone + NADP+ + H2O
-
-
-
-
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
mechanism
-
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
benzene rings of Phe85, Phe87, Tyr397, as well as Tyr216 and the positive charge of Arg235 are important for activity and stabilization of the active site, reaction mechanism
-
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
direct reduction by NADPH of the flavin at the active site without requirement of intermediate formation
-
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
tetrachlorobenzoquinone reductase PcpD reaction mechanism
-
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dehalogenation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
KEGG
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
pentachlorophenol,NADPH:oxygen oxidoreductase (hydroxylating, dechlorinating)
A flavoprotein (FAD). The enzyme displaces a diverse range of substituents from the 4-position of polyhalogenated phenols but requires that a halogen substituent be present at the 2-position [2]. If C-4 carries a halogen substituent, reaction 1 is catalysed; if C-4 is unsubstituted, reaction 2 is catalysed.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
124148-88-5
-
136111-57-4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3,4,5-tetrachlorophenol + NADPH + O2
2,3,5-trichlorohydroquinone + Cl- + NADP+ + H2O
-
-
-
-
?
2,3,5,6-tetrachlorophenol + 2 NADPH + H+ + O2
2,3,5,6-tetrachloro-p-hydroquinone + 2 NADP+ + chloride + H2O
-
-
-
-
?
2,3,5,6-tetrachlorophenol + NADPH + O2
2,3,5,6-tetrachloro-p-hydroquinone + NADP+
-
-
-
?
2,3,6-trichlorophenol + NADPH + O2
2,3,6-trichloro-p-hydroquinone + NADP+ + H2O
-
-
-
?
2,4,6-tribromophenol + NADPH + O2
2,6-dibromo-p-hydroquinone + Br- + NADP+ + H2O
-
low activity
-
?
2,6-dibromo-4-nitrophenol + NADPH + O2
2,6-dibromo-p-hydroquinone + NO2- + NADP+ + H2O
-
low activity
-
?
2,6-dibromophenol + NADPH + O2
2,6-dibromo-p-hydroquinone + NADP+ + H2O
-
-
-
?
2,6-dichlorophenol + NADPH + H+ + O2
2,6-dichloro-p-hydroquinone + NADP+ + H2O
-
-
-
-
?
2,6-dichlorophenol + NADPH + O2
2,6-dichloro-p-hydroquinone + NADP+ + H2O
-
-
-
?
3,4,5-trichlorophenol + 2 NADPH + H+ + O2
3,5-dichloro-p-hydroquinone + 2 NADP+ + chloride + H2O
-
-
-
-
?
3,5-dibromo-4-hydroxybenzonitrile + 2 NADPH + 2 H+ + O2
2,6-dibromo-p-hydroquinone + CN- + 2 NADP+ + H2O
-
-
-
?
3,5-dichlorophenol + NADPH + H+ + O2
3,5-dichloro-p-hydroquinone + NADP+ + H2O
-
-
-
-
?
4-amino-2,6-dichlorophenol + NADPH + O2
2,6-dichloro-p-hydroquinone + NADP+ + NH2OH
-
low activity
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorobenzoquinone + 2 NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + chloride + H2O
-
product is not tetrachlorobenzoquinone as previously thought, ethyl acetate and glutathione can shift the redox-equilibrium in favor of tetrachlorobenzoquinone, room temperature (23-25°C), 50 mM phosphate buffer, pH 7.0, while with NADPH tetrachlorobenzoquinone is reduced to tetrachlorohydroquinone (with 0.5-1 mol NADPH/mol tetrachlorobenzoquinone 50-65% are reduced, with 2.5 mol or more almost complete reduction occurs), deionized distilled water, pH 6.8 (due to interference of phosphate or Tris-HCl buffer with the detection of tetrachlorohydroquinone)
-
?
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NAD+ + chloride + H2O
-
-
-
-
r
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
2,4,6-trichlorophenol + NADPH + O2
2,6-dichloro-p-hydroquinone + Cl- + NADP+ + H2O
-
-
-
-
?
2,4,6-trichlorophenol + NADPH + O2
2,6-dichloro-p-hydroquinone + Cl- + NADP+ + H2O
-
low activity
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
-
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
wild-type and transformed E. coli cells
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
wild-type and mutant strains
-
?
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
Bacillus tropicus AOA-CPS1
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
degradation of pentachlorophenol
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
the enzyme is an unusually slow flavin-dependent monooxygenase (kcat = 0.02/s) that converts pentachlorophenol to a highly reactive product, tetrachlorobenzoquinone. By stopped-flow spectroscopy, it is shown that the steps up to and including formation of tetrachlorobenzoquinone are rapid (5-30/s). Before products can be released from the active site, the strongly oxidizing tetrachlorobenzoquinone abstracts an electron from a donor at the active site, possibly a cysteine residue, resulting in an off-pathway diradical state that only slowly reverts to an intermediate capable of completing the catalytic cycle
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
oxygen and NADPH are essential
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the parahydroxylation of PCP to tetrachlorohydroquinone, pentachlorophenol degradation pathway, overview
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the parahydroxylation of PCP to tetrachlorohydroquinone, pentachlorophenol degradation pathway, overview
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the parahydroxylation of PCP to tetrachlorohydroquinone, pentachlorophenol degradation pathway, overview
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
?
pentachlorophenol + NADPH + H+ + O2
tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
-
-
-
ir
pentachlorophenol + NADPH + H+ + O2
tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
first step of pentachlorophenol degradation pathway
-
-
ir
pentachlorophenol + NADPH + H+ + O2
tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
tetrachlorohydroquinone + NADP+ + chloride + H2O
-
pentachlorophenol degradation
-
-
?
pentachlorophenol + NADPH + H+ + O2
tetrachlorohydroquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
tetrachlorohydroquinone + NADP+ + chloride + H2O
-
pentachlorophenol degradation
-
-
?
tetrachlorobenzoquinone + NADPH
tetrachlorohydroquinone + NADP+
-
-
-
-
?
tetrachlorobenzoquinone + NADPH
tetrachlorohydroquinone + NADP+
-
second step in pentachlorophenol degradation, physiological role in association with pentachloro monooxygenase, overview
-
-
?
triiodophenol + NADPH + O2
diiodohydroquinone + iodide + NADP+ + H2O
-
-
-
-
?
triiodophenol + NADPH + O2
diiodohydroquinone + iodide + NADP+ + H2O
-
-
-
?
additional information
?
-
-
no activity with 3,4,5-trichlorophenol, 2,4-dichlorophenol, 3,4-dichlorophenol, 4-chlorophenol
-
-
?
additional information
?
-
-
enzyme CpsB from Bacillus tropicus reacts with PCP to trap 1,4-tetrachlorobenzoquinone (Tet-CBQ) formed as 2,3,5,6-tetrakis[(2-hydroxyethyl)thio]-1,4-hydroquinone (THTH)
-
-
-
additional information
?
-
Bacillus tropicus AOA-CPS1
-
enzyme CpsB from Bacillus tropicus reacts with PCP to trap 1,4-tetrachlorobenzoquinone (Tet-CBQ) formed as 2,3,5,6-tetrakis[(2-hydroxyethyl)thio]-1,4-hydroquinone (THTH)
-
-
-
additional information
?
-
-
broad substrate specificity, hydroxylation in para-position
-
-
?
additional information
?
-
enzyme also removes amino, cyano and nitro groups from the para-position to the hydroxyl group of the phenol
-
-
?
additional information
?
-
-
enzyme also removes amino, cyano and nitro groups from the para-position to the hydroxyl group of the phenol
-
-
?
additional information
?
-
-
overview: enzyme catalyzes a primary attack on a broad range of substituted phenols, hydroxylating the para position and removing halogen, nitro, amino and cyano groups to produce halide, nitrite, hydroxylamine and cyanide respectively
-
-
?
additional information
?
-
-
no activity with 3,4,5-trichlorophenol, 3,5-dichlorophenol
-
-
?
additional information
?
-
-
broad substrate and catalytic reaction range
-
-
?
additional information
?
-
-
biodegradation of the herbicide bromoxynil, i.e. 3,5-dibromo-4-hydroxybenzonitrile
-
-
?
additional information
?
-
-
lateral gene transfer and the origin of the pentachlorophenol pathway, overview
-
-
?
additional information
?
-
-
enzyme expression in response to pentachlorophenol hydroxylase PcpB
-
-
?
additional information
?
-
-
lateral gene transfer and the origin of the pentachlorophenol pathway, overview
-
-
?
additional information
?
-
-
enzyme exhibits substantial uncoupling. The C4a-hydroxyflavin intermediate, instead of hydroxylating the substrate, can decompose to produce H2O2 in a futile cycle that consumes NADPH. The extent of uncoupling varies from 0 to 100% with different substrates. The extent of uncoupling is increased by the presence of bulky substituents at position 3, 4, or 5 and decreased by the presence of a chlorine in the ortho position. The effectiveness of pentachlorophenol hydroxylase is additionally hindered by its promiscuous activity with tetrachlorohydroquinone, a downstream metabolite in the degradation pathway. The conversion of tetrachlorohydroquinone to tetrachlorobenzoquinone reverses flux through the pathway. Substantial uncoupling also occurs during the reaction with tetrachlorohydroquinone
-
-
?
additional information
?
-
broad substrate specificity, hydroxylation in para-position
-
-
?
additional information
?
-
-
lateral gene transfer and the origin of the pentachlorophenol pathway, overview
-
-
?
additional information
?
-
broad substrate specificity, hydroxylation in para-position
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + chloride + H2O
-
product is not tetrachlorobenzoquinone as previously thought, ethyl acetate and glutathione can shift the redox-equilibrium in favor of tetrachlorobenzoquinone, room temperature (23-25°C), 50 mM phosphate buffer, pH 7.0, while with NADPH tetrachlorobenzoquinone is reduced to tetrachlorohydroquinone (with 0.5-1 mol NADPH/mol tetrachlorobenzoquinone 50-65% are reduced, with 2.5 mol or more almost complete reduction occurs), deionized distilled water, pH 6.8 (due to interference of phosphate or Tris-HCl buffer with the detection of tetrachlorohydroquinone)
-
?
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
degradation of pentachlorophenol
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
tetrachlorobenzoquinone + NADPH
tetrachlorohydroquinone + NADP+
-
second step in pentachlorophenol degradation, physiological role in association with pentachloro monooxygenase, overview
-
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
-
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
wild-type and transformed E. coli cells
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one
-
?
pentachlorophenol + 2 NADPH + H+ + O2
tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
wild-type and mutant strains
-
?
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
-
-
-
?
pentachlorophenol + NADH + H+ + O2
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
Bacillus tropicus AOA-CPS1
-
-
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the parahydroxylation of PCP to tetrachlorohydroquinone, pentachlorophenol degradation pathway, overview
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the parahydroxylation of PCP to tetrachlorohydroquinone, pentachlorophenol degradation pathway, overview
-
-
?
pentachlorophenol + NADPH + H+ + O2
2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
initial enzyme in the PCP catabolic pathway of numerous sphingomonads, PCP-4-monooxygenase (PcpB), catalyzes the parahydroxylation of PCP to tetrachlorohydroquinone, pentachlorophenol degradation pathway, overview
-
-
?
pentachlorophenol + NADPH + H+ + O2
tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
-
-
-
ir
pentachlorophenol + NADPH + H+ + O2
tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
first step of pentachlorophenol degradation pathway
-
-
ir
pentachlorophenol + NADPH + H+ + O2
tetrachlorohydroquinone + NADP+ + chloride + H2O
-
pentachlorophenol degradation
-
-
?
pentachlorophenol + NADPH + H+ + O2
tetrachlorohydroquinone + NADP+ + chloride + H2O
-
pentachlorophenol degradation
-
-
?
additional information
?
-
-
biodegradation of the herbicide bromoxynil, i.e. 3,5-dibromo-4-hydroxybenzonitrile
-
-
?
additional information
?
-
-
lateral gene transfer and the origin of the pentachlorophenol pathway, overview
-
-
?
additional information
?
-
-
enzyme expression in response to pentachlorophenol hydroxylase PcpB
-
-
?
additional information
?
-
-
lateral gene transfer and the origin of the pentachlorophenol pathway, overview
-
-
?
additional information
?
-
-
lateral gene transfer and the origin of the pentachlorophenol pathway, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
dithiothreitol can not be used as electron donor
-
FAD
-
recombinant enzyme has no FAD, activity only in presence of added FAD
NADPH
-
2 mol of NADPH per mol of halogenated substrate, preferred electron donor
NADPH
-
elimination of 1 mol of a halogen, nitro, or cyano group requires 2 mol of NADPH, 1 mol of NADPH is required to remove 1 mol of an amino group or hydrogen
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Fe3+
-
the catalytic active site of the enzyme has a Fe metal ion-binding centre in contact with residues His124, Glu125, His129 and Glu214. Fe3+ does not activate the enzyme activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
tetrachlorobenzoquinone
-
inactivates the enzyme, feedback control
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
pentachlorphenol induces enzyme expression
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0005
Pentachlorophenol
-
pH 7.0, 25°C, recombinant wild-type enzyme
0.0008
Pentachlorophenol
-
pH 7.0, 25°C, recombinant mutant R235K