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Literature summary for 1.14.13.22 extracted from

  • Zhang, Y.; Wu, Y.; Xu, N.; Zhao, Q.; Yu, H.; Xu, J.
    Engineering of cyclohexanone monooxygenase for the enantioselective synthesis of (S)-omeprazole (2019), ACS Sust. Chem. Eng., 7, 7218-7226 .
No PubMed abstract available
Search for more literature for 1.14.13.22

Application

Application Comment Organism
analysis effective halo-based selection method for mutant proteins using the solubility difference between the substrate (omeprazole sulfide) and product (esomeprazole) Acinetobacter calcoaceticus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of variant F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L complexed with FAD, to 2.2 A resolution Acinetobacter calcoaceticus

Protein Variants

Protein Variants Comment Organism
F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L substrate specificity is markedly altered from substrate cyclohexanone toward the desired bulky substrate omeprazole sulfide despite an extremely poor activity Acinetobacter calcoaceticus
L143P/F246Y/K269E/K326C/L426F/F432L/T433A/L435S/S438I/F505L/N386S/I388K/M390I/E488K/S489C/W490R 50fold higher activity with substrate substrate omeprazole than mutant F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methoxy-2-methylthio-1H-benzimidazole + NADPH + H+ + O2 poor substrate for wild-type Acinetobacter calcoaceticus ?
-
 ?
cyclohexanone + NADPH + H+ + O2
-
 Acinetobacter calcoaceticus hexano-6-lactone + NADP+ + H2O
-
 ?
omeprazole sulfide + NADPH + H+ + O2 no substrae for wild-type Acinetobacter calcoaceticus (S)-omeprazole + NADP+ + H2O
-
 ?
thioanisole + NADPH + H+ + O2
-
 Acinetobacter calcoaceticus ?
-
 ?