BRENDA - Enzyme Database show
show all sequences of 1.14.13.209

Novel pathway of salicylate degradation by Streptomyces sp. strain WA46

Ishiyama, D.; Vujaklija, D.; Davies, J.; Appl. Environ. Microbiol. 70, 1297-1306 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Streptomyces lividans TK64 and Escherichia coli DH10B cells
Streptomyces sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56600
-
x * 56600, calculated from amino acid sequence
Streptomyces sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-hydroxybenzoyl-CoA + NADH + H+ + O2
Streptomyces sp.
-
gentisyl-CoA + NAD+ + H2O
-
-
?
2-hydroxybenzoyl-CoA + NADH + H+ + O2
Streptomyces sp. WA46
-
gentisyl-CoA + NAD+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
Q7X281
-
-
Streptomyces sp. WA46
Q7X281
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxybenzoyl-CoA + NADH + H+ + O2
-
733151
Streptomyces sp.
gentisyl-CoA + NAD+ + H2O
-
-
-
?
2-hydroxybenzoyl-CoA + NADH + H+ + O2
-
733151
Streptomyces sp. WA46
gentisyl-CoA + NAD+ + H2O
-
-
-
?
additional information
direct conversion of salicylate to gentisate is not observed
733151
Streptomyces sp.
?
-
-
-
-
additional information
direct conversion of salicylate to gentisate is not observed
733151
Streptomyces sp. WA46
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 56600, calculated from amino acid sequence
Streptomyces sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
required, neither NADPH nor FAD can replace NADH
Streptomyces sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Streptomyces lividans TK64 and Escherichia coli DH10B cells
Streptomyces sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
required, neither NADPH nor FAD can replace NADH
Streptomyces sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56600
-
x * 56600, calculated from amino acid sequence
Streptomyces sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-hydroxybenzoyl-CoA + NADH + H+ + O2
Streptomyces sp.
-
gentisyl-CoA + NAD+ + H2O
-
-
?
2-hydroxybenzoyl-CoA + NADH + H+ + O2
Streptomyces sp. WA46
-
gentisyl-CoA + NAD+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxybenzoyl-CoA + NADH + H+ + O2
-
733151
Streptomyces sp.
gentisyl-CoA + NAD+ + H2O
-
-
-
?
2-hydroxybenzoyl-CoA + NADH + H+ + O2
-
733151
Streptomyces sp. WA46
gentisyl-CoA + NAD+ + H2O
-
-
-
?
additional information
direct conversion of salicylate to gentisate is not observed
733151
Streptomyces sp.
?
-
-
-
-
additional information
direct conversion of salicylate to gentisate is not observed
733151
Streptomyces sp. WA46
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56600, calculated from amino acid sequence
Streptomyces sp.
Other publictions for EC 1.14.13.209
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733151
Ishiyama
Novel pathway of salicylate de ...
Streptomyces sp., Streptomyces sp. WA46
Appl. Environ. Microbiol.
70
1297-1306
2004
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