BRENDA - Enzyme Database show
show all sequences of 1.14.13.101

Independent recruitment of a flavin-dependent monooxygenase for safe accumulation of sequestered pyrrolizidine alkaloids in grasshoppers and moths

Wang, L.; Beuerle, T.; Timbilla, J.; Ober, D.; PLoS ONE 7, e31796 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Zonocerus variegatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00108
-
senecionine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.00983
-
atropine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.01193
-
monocrotaline
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.2639
-
heliotrine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.8376
-
phalaenopsine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47793
-
2 * 47793, calculated from amino acid sequence
Zonocerus variegatus
91200
-
gel filtration
Zonocerus variegatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
senecionine + NADPH + H+ + O2
Zonocerus variegatus
most efficient substrate
senecionine N-oxide + NADP+ + H2O
-
-
?
seneciphylline + NADPH + H+ + O2
Zonocerus variegatus
second best substrate
seneciphylline N-oxide + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zonocerus variegatus
-
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-NTA agarose column chromatography
Zonocerus variegatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
atropine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
atropine N-oxide + NADP+ + H2O
-
-
-
?
axillaridine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
axillaridine N-oxide + NADP+ + H2O
-
-
-
?
axillarine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
axillarine N-oxide + NADP+ + H2O
-
-
-
?
cysteamine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
cysteamine N-oxide + NADP+ + H2O
-
-
-
?
heliotrine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
heliotrine N-oxide + NADP+ + H2O
-
-
-
?
indicine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
indicine N-oxide + NADP+ + H2O
-
-
-
?
monocrotaline + NADPH + H+ + O2
-
728567
Zonocerus variegatus
monocrotaline N-oxide + NADP+ + H2O
-
-
-
?
additional information
no activity with senkirkine, heliotridine, supinidine, ephedrine, dimethylaniline, L-cysteine, hydroxylamine, and glutathione
728567
Zonocerus variegatus
?
-
-
-
-
nicotine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
nicotine N-oxide + NADP+ + H2O
-
-
-
?
phalaenopsine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
phalaenopsine N-oxide + NADP+ + H2O
-
-
-
?
retronecine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
retronecine N-oxide + NADP+ + H2O
-
-
-
?
retrorsine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
retrorsine N-oxide + NADP+ + H2O
-
-
-
?
rinderine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
rinderine N-oxide + NADP+ + H2O
-
-
-
?
sarracine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
sarracine N-oxide + NADP+ + H2O
-
-
-
?
senecionine + NADPH + H+ + O2
most efficient substrate
728567
Zonocerus variegatus
senecionine N-oxide + NADP+ + H2O
-
-
-
?
seneciphylline + NADPH + H+ + O2
second best substrate
728567
Zonocerus variegatus
seneciphylline N-oxide + NADP+ + H2O
-
-
-
?
senecivernine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
senecivernine N-oxide + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 47793, calculated from amino acid sequence
Zonocerus variegatus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
42
-
-
Zonocerus variegatus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.12
-
heliotrine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.13
-
phalaenopsine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.37
-
atropine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.96
-
monocrotaline
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.99
-
senecionine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
in potassium phosphate buffer (10 mM), Tris/HCl buffer (10 mM), and in glycine/NaOH buffer (100 mM)
Zonocerus variegatus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Zonocerus variegatus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Zonocerus variegatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Zonocerus variegatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00108
-
senecionine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.00983
-
atropine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.01193
-
monocrotaline
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.2639
-
heliotrine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.8376
-
phalaenopsine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47793
-
2 * 47793, calculated from amino acid sequence
Zonocerus variegatus
91200
-
gel filtration
Zonocerus variegatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
senecionine + NADPH + H+ + O2
Zonocerus variegatus
most efficient substrate
senecionine N-oxide + NADP+ + H2O
-
-
?
seneciphylline + NADPH + H+ + O2
Zonocerus variegatus
second best substrate
seneciphylline N-oxide + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA agarose column chromatography
Zonocerus variegatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
atropine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
atropine N-oxide + NADP+ + H2O
-
-
-
?
axillaridine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
axillaridine N-oxide + NADP+ + H2O
-
-
-
?
axillarine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
axillarine N-oxide + NADP+ + H2O
-
-
-
?
cysteamine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
cysteamine N-oxide + NADP+ + H2O
-
-
-
?
heliotrine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
heliotrine N-oxide + NADP+ + H2O
-
-
-
?
indicine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
indicine N-oxide + NADP+ + H2O
-
-
-
?
monocrotaline + NADPH + H+ + O2
-
728567
Zonocerus variegatus
monocrotaline N-oxide + NADP+ + H2O
-
-
-
?
additional information
no activity with senkirkine, heliotridine, supinidine, ephedrine, dimethylaniline, L-cysteine, hydroxylamine, and glutathione
728567
Zonocerus variegatus
?
-
-
-
-
nicotine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
nicotine N-oxide + NADP+ + H2O
-
-
-
?
phalaenopsine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
phalaenopsine N-oxide + NADP+ + H2O
-
-
-
?
retronecine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
retronecine N-oxide + NADP+ + H2O
-
-
-
?
retrorsine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
retrorsine N-oxide + NADP+ + H2O
-
-
-
?
rinderine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
rinderine N-oxide + NADP+ + H2O
-
-
-
?
sarracine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
sarracine N-oxide + NADP+ + H2O
-
-
-
?
senecionine + NADPH + H+ + O2
most efficient substrate
728567
Zonocerus variegatus
senecionine N-oxide + NADP+ + H2O
-
-
-
?
seneciphylline + NADPH + H+ + O2
second best substrate
728567
Zonocerus variegatus
seneciphylline N-oxide + NADP+ + H2O
-
-
-
?
senecivernine + NADPH + H+ + O2
-
728567
Zonocerus variegatus
senecivernine N-oxide + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 47793, calculated from amino acid sequence
Zonocerus variegatus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
42
-
-
Zonocerus variegatus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.12
-
heliotrine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.13
-
phalaenopsine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.37
-
atropine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.96
-
monocrotaline
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.99
-
senecionine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
in potassium phosphate buffer (10 mM), Tris/HCl buffer (10 mM), and in glycine/NaOH buffer (100 mM)
Zonocerus variegatus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.155
-
phalaenopsine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.454
-
heliotrine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
37.64
-
atropine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
80.5
-
monocrotaline
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
916.7
-
senecionine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.155
-
phalaenopsine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
0.454
-
heliotrine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
37.64
-
atropine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
80.5
-
monocrotaline
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
916.7
-
senecionine
in 100 mM glycine/NaOH buffer pH 9.0, at 37C
Zonocerus variegatus
Other publictions for EC 1.14.13.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728567
Wang
Independent recruitment of a f ...
Zonocerus variegatus
PLoS ONE
7
e31796
2012
-
-
1
-
-
-
-
5
-
-
2
2
-
1
-
-
1
-
-
-
-
-
17
1
1
-
-
5
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
2
2
-
-
-
1
-
-
-
-
17
1
1
-
-
5
1
-
-
-
-
-
-
-
5
5
706452
Sehlmeyer
Flavin-dependent monooxygenase ...
Apantesis nevadensis geneura, Tyria jacobaeae
PLoS ONE
5
e10435
2010
-
-
2
-
-
-
-
-
-
-
-
1
-
10
-
-
-
-
-
2
1
-
10
-
1
-
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
653688
Naumann
Evolutionary recruitment of a ...
Tyria jacobaeae
Proc. natl. Acad. Sci. USA
99
6085-6090
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
8
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689412
Chang
-
Solubilization and characteriz ...
Crotalaria scassellatii
Phytochemistry
49
1859-1866
1998
3
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
651040
Lindigkeit
The two faces of pyrrolizidine ...
Amerila phaedra, Arctia caja, Arctia intercalaris, Callimorpha dominula, Creatonotos transiens, Idea leuconoe, no activity in Spodoptera littoralis, Tyria jacobaeae, Zonocerus variegatus
Eur. J. Biochem.
245
626-636
1997
-
-
-
-
-
-
-
10
1
-
2
-
-
17
-
-
1
-
-
8
1
-
40
1
1
-
-
-
1
-
-
4
-
1
-
-
-
-
4
-
-
-
-
-
-
10
1
-
2
-
-
-
-
1
-
8
1
-
40
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-