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Literature summary for 1.14.12.23 extracted from

  • Singh, D.; Kumari, A.; Ramaswamy, S.; Ramanathan, G.
    Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2 (2014), Biochem. Biophys. Res. Commun., 445, 36-42.
    View publication on PubMed
Search for more literature for 1.14.12.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Diaphorobacter sp. DS2

Protein Variants

Protein Variants Comment Organism
additional information individual expression of each subunit and separate purification. The active recombinant enzyme can be reconstituted in vitro by mixing all three purified subunits Diaphorobacter sp. DS2

Organism

Organism UniProt Comment Textmining
Diaphorobacter sp. DS2 M9PTH9 and M9PW10 and M9PSS0 and M9PV03 M9PTH9 i.e. ferredoxin reductase subunit MntAa, M9PW10 i.e. oxygenase alpha subunit MntAc, M9PSS0 i.e. ferredoxin subunit MntAb, M9PV03 i.e. oxygenase beta subunit MntAd
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dinitrotoluene + NADH + O2
-
 Diaphorobacter sp. DS2 3-methyl-4-nitrocatechol + nitrite + NAD+ 84% of the activity with 3-nitrotoluene ?
2-chloronitrobenzene + NADH + O2
-
 Diaphorobacter sp. DS2 3-chlorocatechol + nitrite + NAD+ 80% of the activity with 3-nitrotoluene ?
2-nitrotoluene + NADH + O2
-
 Diaphorobacter sp. DS2 3-methylcatechol + nitrite + NAD+ 71% of the activity with 3-nitrotoluene. Products are 91% 3-methylcatechol, 9% 3-nitrobenzyl alcohol ?
3-nitrotoluene + NADH + O2
-
 Diaphorobacter sp. DS2 3-methylcatechol + 4-methylcatechol + nitrite + NAD+ products are 54% 3-methylcatechol, 39% 4-methylcatechol, 7% 3-nitrobenzyl alcohol ?
nitrobenzene + NADH + O2
-
 Diaphorobacter sp. DS2 catechol + nitrite + NAD+ 78% of the activity with 3-nitrotoluene ?

Synonyms

Synonyms Comment Organism
MntAa
-
 Diaphorobacter sp. DS2
MntAb
-
 Diaphorobacter sp. DS2
MntAc
-
 Diaphorobacter sp. DS2
MntAd
-
 Diaphorobacter sp. DS2