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Literature summary for 1.14.11.67 extracted from
- Control of transposon activity by a histone H3K4 demethylase in rice (2013), Proc. Natl. Acad. Sci. USA, 110, 1953-1958 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene JMJ703, DNA and amino acid sequence determination and analysis, genotyping, functional overexpression of YFP-HA-tagged JMJ703 in Nicotiana tabacum cells via transfection with Agrobacterium tumefaciens strain EHA105 | Oryza sativa Japonica Group |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression of JMJ703-YFP-HA reduces the levels of H3K4me3/2/1 in vivo. T-DNA insertion disruption of JMJ703 transcription, four of the validated target genes show the decreased CpG methylation at their 5' regions with the increased H3K4me3 in the mutant compared with wild-type. Loss-of-function mutant jmj703 derepresses thousands of genes, especially those involved in chromatin assembly. Mutant jmj703 displays pleiotropic defects that could be due to ectopic expression or upregulation of direct targets of JMJ703 | Oryza sativa Japonica Group |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Oryza sativa Japonica Group | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Oryza sativa Japonica Group | two LINE elements, Karma and its N-terminal truncation, are identified as direct targets of JMJ703 in epigenetic regulation by demethylation. Karma is directly associated with JMJ703 | ? | - |
? |  |
| [histone H3]-N6,N6,N6-trimethyl-L-lysine 4 + 2-oxoglutarate + O2 | Oryza sativa Japonica Group | - |
[histone H3]-N6,N6-dimethyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
| [histone H3]-N6,N6-drimethyl-L-lysine 4 + 2-oxoglutarate + O2 | Oryza sativa Japonica Group | - |
[histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
| [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 | Oryza sativa Japonica Group | - |
[histone H3]-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa Japonica Group | Q53WJ1 | cv. Dongjin | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Oryza sativa Japonica Group | - |
| leaf | - |
Oryza sativa Japonica Group | - |
| seedling | - |
Oryza sativa Japonica Group | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | two LINE elements, Karma and its N-terminal truncation, are identified as direct targets of JMJ703 in epigenetic regulation by demethylation. Karma is directly associated with JMJ703 | Oryza sativa Japonica Group | ? | - |
? | |
| additional information | the enzyme JMJ703 is specific for H3K4me3/me2/me1 residues, no activity on H3K9me3/2/1, H3K27me3/2/1 and H3K36me3/2/1 | Oryza sativa Japonica Group | ? | - |
? | |
| [histone H3]-N6,N6,N6-trimethyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Oryza sativa Japonica Group | [histone H3]-N6,N6-dimethyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
| [histone H3]-N6,N6-drimethyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Oryza sativa Japonica Group | [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
| [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 | - |
Oryza sativa Japonica Group | [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| histone H3K4 demethylase | - |
Oryza sativa Japonica Group |
| histone H3K4-specific demethylase | - |
Oryza sativa Japonica Group |
| JMJ703 | - |
Oryza sativa Japonica Group |
| lysine-specific demethylase | - |
Oryza sativa Japonica Group |
| lysine-specific demethylase JMJ703 | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryza sativa Japonica Group |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Oryza sativa Japonica Group |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | JMJ703 is the homolog of the Arabidopsis thaliana H3K4 demethylase JMJ14, which is involved in flowering time regulation and gene silencing | Oryza sativa Japonica Group |
| malfunction | overexpression of JMJ703-YFP-HA reduces the levels of H3K4me3/2/1 in vivo. JMJ703 loss-of-function mutant displays pleiotropic phenotypes. Impaired JMJ703 activity leads to elevated levels of H3K4me3, the misregulation of numerous endogenous genes, and the transpositional reactivation of two families of non-LTR retrotransposons. But loss of JMJ703 does not affect transposable elements (such as Tos17) previously found to be silenced by other epigenetic pathways. Overexpression of JMJ703-YFP-HA reduces the levels of H3K4me3/2/1 in vivo. Karma, a non-LTR LINE-type retrotransposon (LOC_Os11g44750, in a chromosome niche depleted in H3K4me3), shows upregulated gene expression and significantly increased association with H3K4me3 in jmj703. In T-DNA insertion disruption mutants of JMJ703, four of the validated target genes show the decreased CpG methylation at their 5' regions with the increased H3K4me3 in the mutant compared to wild-type | Oryza sativa Japonica Group |
| metabolism | model of the distinct mechanisms controlling transposable element silencing, overview. A transposable element located in a euchromatic region containing active genes associated with H3K4me3 is silenced by additional repressive marks like H3K9me2. If H3K9me2 methyltransferase is impaired, the repressive marks are removed and the transposable element is activated. A transposable element located in a heterochromatic region is silenced by active H3K4me3 demethylase | Oryza sativa Japonica Group |
| physiological function | JMJ703 is an active H3K4-specific demethylase required for transposable elements silencing, overview. The removal of active histone modifications is involved in transposable elements silencing and different subsets of transposable elements may be regulated by distinct epigenetic pathways. Histone demethylation is a unique mechanism to control retrotransposon activity. Effects of JMJ703 on H3K4me3 and gene expression, detailed overview | Oryza sativa Japonica Group |
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Release 2023.1 (January 2023)
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