Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens | |
IOX1 | competitive inhibition | Homo sapiens | |
additional information | the presence of S-adenosyl-L-homocysteine, NaCl, MgCl2, dimethylsulfoxide, and Triton X-100 does not influence enzyme activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000192 | - |
5'-UACACUCGAUCUGG(m6A)CUAAAGCUGCUC-3'-biotin | at pH 8.0 and 37°C | Homo sapiens | |
0.0025 | - |
2-oxoglutarate | at pH 8.0 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on, 0.02 mM Fe2+ used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-methyladenine in mRNA + 2-oxoglutarate + O2 | Homo sapiens | - |
adenine in mRNA + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DE52 column chromatography, Ni-NTA column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-UACACUCGAUCUGG(m6A)CUAAAGCUGCUC-3'-biotin + 2-oxoglutarate + O2 | - |
Homo sapiens | 5'-UACACUCGAUCUGGCUAAAGCUGCUC-3'-biotin + formaldehyde + succinate + CO2 | - |
? | |
N6-methyladenine in mRNA + 2-oxoglutarate + O2 | - |
Homo sapiens | adenine in mRNA + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALKBH5 | - |
Homo sapiens |
m6A-RNA demethylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-oxoglutarate | at pH 8.0 and 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
2-oxoglutarate | at pH 8.0 and 37°C | Homo sapiens |