BRENDA - Enzyme Database
show all sequences of 1.13.11.54

Acireductone dioxygenase 1 (ARD1) is an effector of the heterotrimeric G protein beta subunit in Arabidopsis

Friedman, E.; Wang, H.; Jiang, K.; Perovic, I.; Deshpande, A.; Pochapsky, T.; Temple, B.; Hicks, S.; Harden, T.; Jones, A.; J. Biol. Chem. 286, 30107-30118 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Arabidopsis thaliana G protein beta
possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD1–4 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
usage of three tDNA insertion mutant alleles, ard1-1 (SALK_119327), ard1-2 (GABI_595C04), and ard1-3 (SALK_034308).
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, acireductone dioxygenase 1 is an active metalloenzyme, Fe2+ is active site bound
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
Arabidopsis thaliana
-
4-(methylthio)-2-oxobutanoate + formate
-
-
?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
Arabidopsis thaliana Columbia-0
-
4-(methylthio)-2-oxobutanoate + formate
-
-
?
additional information
Arabidopsis thaliana
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
?
-
-
-
additional information
Arabidopsis thaliana Columbia-0
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q0WSS4
-
-
Arabidopsis thaliana Columbia-0
Q0WSS4
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged acireductone dioxygenase 1 and GST-tagged acireductone dioxygenase 1 by nickel affinity and glutathione affinity chromatographies, respectively
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
725438
Arabidopsis thaliana
4-(methylthio)-2-oxobutanoate + formate
-
-
-
?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
725438
Arabidopsis thaliana Columbia-0
4-(methylthio)-2-oxobutanoate + formate
-
-
-
?
additional information
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
725438
Arabidopsis thaliana
?
-
-
-
-
additional information
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
725438
Arabidopsis thaliana Columbia-0
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
homology modeling of acireductone dioxygenase 1, overview
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Arabidopsis thaliana G protein beta
possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD1–4 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
usage of three tDNA insertion mutant alleles, ard1-1 (SALK_119327), ard1-2 (GABI_595C04), and ard1-3 (SALK_034308).
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, acireductone dioxygenase 1 is an active metalloenzyme, Fe2+ is active site bound
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
Arabidopsis thaliana
-
4-(methylthio)-2-oxobutanoate + formate
-
-
?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
Arabidopsis thaliana Columbia-0
-
4-(methylthio)-2-oxobutanoate + formate
-
-
?
additional information
Arabidopsis thaliana
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
?
-
-
-
additional information
Arabidopsis thaliana Columbia-0
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged acireductone dioxygenase 1 and GST-tagged acireductone dioxygenase 1 by nickel affinity and glutathione affinity chromatographies, respectively
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
725438
Arabidopsis thaliana
4-(methylthio)-2-oxobutanoate + formate
-
-
-
?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
725438
Arabidopsis thaliana Columbia-0
4-(methylthio)-2-oxobutanoate + formate
-
-
-
?
additional information
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
725438
Arabidopsis thaliana
?
-
-
-
-
additional information
acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview
725438
Arabidopsis thaliana Columbia-0
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
homology modeling of acireductone dioxygenase 1, overview
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
additional information
identification of potential effectors in the Arabidopsis thaliana G protein beta signaling pathway by an activation tagging approach to randomly create dominant suppressors of agb1-2, overview. Homology modeling of acireductone dioxygenase 1, overview
Arabidopsis thaliana
physiological function
one function of acireductone dioxygenases is to operate in the methionine salvage pathway. Acireductone dioxygenase 1 physically interacts with Arabidopsis thaliana G protein beta, and acireductone dioxygenase enzymatic activity is stimulated by Arabidopsis thaliana G protein beta in vitro. Overexpression of acireductone dioxygenase 1 suppresses the 2-day-old etiolated phenotype of agb1-2. Acireductone dioxygenase 1 and Arabidopsis thaliana G protein beta both control embryonic hypocotyl length by modulating cell division. They also may contribute to the production of ethylene, a product of the methionine salvage pathway. Arabidopsis thaliana G protein beta regulates acireductone dioxygenase 1 activity to control cell division, possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
additional information
identification of potential effectors in the Arabidopsis thaliana G protein beta signaling pathway by an activation tagging approach to randomly create dominant suppressors of agb1-2, overview. Homology modeling of acireductone dioxygenase 1, overview
Arabidopsis thaliana
physiological function
one function of acireductone dioxygenases is to operate in the methionine salvage pathway. Acireductone dioxygenase 1 physically interacts with Arabidopsis thaliana G protein beta, and acireductone dioxygenase enzymatic activity is stimulated by Arabidopsis thaliana G protein beta in vitro. Overexpression of acireductone dioxygenase 1 suppresses the 2-day-old etiolated phenotype of agb1-2. Acireductone dioxygenase 1 and Arabidopsis thaliana G protein beta both control embryonic hypocotyl length by modulating cell division. They also may contribute to the production of ethylene, a product of the methionine salvage pathway. Arabidopsis thaliana G protein beta regulates acireductone dioxygenase 1 activity to control cell division, possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview
Arabidopsis thaliana
Other publictions for EC 1.13.11.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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742270
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Klebsiella oxytoca
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135
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2
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725438
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Acireductone dioxygenase 1 (AR ...
Arabidopsis thaliana, Arabidopsis thaliana Columbia-0
J. Biol. Chem.
286
30107-30118
2011
1
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1
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1
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1
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4
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6
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1
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4
1
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4
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1
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2
2
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Klebsiella oxytoca, Klebsiella oxytoca ATCC 8724
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47
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2008
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9
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363
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1
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1
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1
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1
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2
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1
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1
-
2
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2
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673849
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Homo sapiens, Saccharomyces cerevisiae, Saccharomyces cerevisiae Y700
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10
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2
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661058
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Klebsiella pneumoniae
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40
6379-6387
2001
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2
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662101
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One protein, two enzymes ...
Klebsiella oxytoca
J. Biol. Chem.
274
1193-1195
1999
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