General Stability | Organism |
---|---|
freezing and thawing changes properties of enzyme, different behavior in anion exchange chromatography | Methanospirillum hungatei |
unstable in prereduced buffer, dramatic decline of activity after 2 h | Methanospirillum hungatei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activating | Methanospirillum hungatei | |
Iron | - |
Methanothermobacter thermautotrophicus | |
Iron | non-heme iron | Methanospirillum hungatei | |
Mg2+ | activating | Methanospirillum hungatei | |
Nickel | - |
Methanothermobacter thermautotrophicus | |
Nickel | - |
Methanospirillum hungatei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30700 | - |
alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy | Methanospirillum hungatei |
50700 | - |
alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy | Methanospirillum hungatei |
720000 | - |
- |
Methanospirillum hungatei |
960000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | Methanothermobacter thermautotrophicus | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | Methanospirillum hungatei | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanospirillum hungatei | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
strain delta H | - |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
- |
Methanospirillum hungatei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.847 | - |
purified enzyme, F420-linked activity | Methanospirillum hungatei |
11.1 | - |
purified enzyme, benzyl viologen-linked activity | Methanospirillum hungatei |
Storage Stability | Organism |
---|---|
liquid nitrogen, in presence of 50% sucrose and 10% glycerol, stable | Methanospirillum hungatei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + benzyl viologen | - |
Methanospirillum hungatei | ? | - |
? | |
H2 + coenzyme F420 | - |
Methanothermobacter thermautotrophicus | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | - |
Methanospirillum hungatei | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanothermobacter thermautotrophicus | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanospirillum hungatei | reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | alpha,beta,gamma 45000 + 31000 + 28000, SDS-PAGE, exact subunit composition not known | Methanothermobacter thermautotrophicus |
multimer | alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy | Methanospirillum hungatei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for added FAD | Methanospirillum hungatei |