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Information on EC 1.12.98.1 - coenzyme F420 hydrogenase
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1.12.98.1 coenzyme F420 hydrogenaseIUBMB Comments
An iron-sulfur flavoprotein (FAD) containing nickel. The enzyme from some sources contains selenocysteine. The enzyme also reduces the riboflavin analogue of F420, flavins and methyl viologen, but to a lesser extent. The hydrogen acceptor coenzyme F420 is a deazaflavin derivative.
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Word Map
- 1.12.98.1
-
methanogen
- methanobacterium
- thermoautotrophicum
-
methanococcus
- hydrogenases
- nife-hydrogenase
- viologens
-
methanogenesis
-
heterodisulfide
-
nickel-containing
- barkeri
- voltae
- fusaro
-
si-face
-
methanothermobacter
- n5,n10-methylenetetrahydromethanopterin
- formicicum
- methylenetetrahydromethanopterin
-
hydrogen-dependent
-
h2-forming
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
f420-reducing hydrogenase, f420h2 dehydrogenase, f420-reducing [nife]-hydrogenase, frhagb-encoded hydrogenase, frhabg, coenzyme f420-dependent hydrogenase, f420-reducing [nife] hydrogenase, 8-hydroxy-5-deazaflavin-reducing hydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
8-hydroxy-5-deazaflavin-reactive hydrogenase
8-hydroxy-5-deazaflavin-reducing hydrogenase
-
-
-
-
coenzyme F420-dependent hydrogenase
-
-
-
-
deazaflavin-reducing hydrogenase
-
-
-
-
EC 1.12.99.1
-
-
formerly
-
F420-reducing hydrogenase
F420-reducing [NiFe]-hydrogenase
FRH
frhAGB-encoded hydrogenase
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
-
hydrogen:(acceptor) oxidoreductase
-
-
-
-
Mbar_A0449
Mbar_A0450
Mbar_A0452
8-hydroxy-5-deazaflavin-reactive hydrogenase
-
-
F420-reducing hydrogenase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
H2 + oxidized coenzyme F420 = reduced coenzyme F420
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
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-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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-
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SYSTEMATIC NAME
IUBMB Comments
hydrogen:coenzyme F420 oxidoreductase
An iron-sulfur flavoprotein (FAD) containing nickel. The enzyme from some sources contains selenocysteine. The enzyme also reduces the riboflavin analogue of F420, flavins and methyl viologen, but to a lesser extent. The hydrogen acceptor coenzyme F420 is a deazaflavin derivative.
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CAS REGISTRY NUMBER
COMMENTARY
9027-05-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
H2 + thioredoxin
reduced thioredoxin
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
the frhAGB-encoded hydrogenase can transfer electrons derived from oxidation of H2 to a protein target by direct contact without the involvement of an electron carrier
-
-
?
oxidized methyl viologen + H2
reduced methyl viologen + H+
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
-
-
-
?
H2 + coenzyme F0
reduced coenzyme F0
-
F0 is 7,8-didemethyl-8-hydroxy-5-deazariboflavin
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
r
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
two alternative pathways, the F420-reducing hydrogenase and the Hmd-Mtd cycle (2-dependent methylenetetrahydromethanopterin dehydrogenase (Hmd) and F420H2-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) together reduce F420 with H2) can function in vivo for the reduction of F420 with H2. Furthermore, during growth on formate the same pathways function in reverse to produce H2 from F420H2. The lack of growth differences between the wild-type and mutant strains on H2 and CO2 suggests that neither pathway for F420 reduction is rate limiting. However, in nature the F420-reducing hydrogenase may constitute the major pathway when sufficient nickel is present, while the Hmd-Mtd cycle may be important when nickel is limiting
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
Methanosarcina barkeri Fusaro / DSM 804
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
F420 is 7,8-didemethyl-8-hydroxy-5-deazaflavin
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
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-
-
?
H2 + coenzyme F420
reduced coenzyme F420
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enzyme of methanogenesis pathway
-
?
H2 + methyl viologen
?
-
binding site for methyl viologen different from F420 binding site as determined by blocking of binding site with antibodies against native enzyme
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-
?
H2 + methyl viologen
?
-
binding site for methyl viologen different from F420 binding site
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-
?
H2 + oxidized benzyl viologen
reduced benzyl viologen
-
-
-
?
H2 + oxidized benzyl viologen
reduced benzyl viologen
-
-
-
?
H2 + oxidized coenzyme F0
reduced coenzyme F20
cofactor F0 i.e. 7,8-didemethy1-8-hydroxy-S-deazaflavin
-
-
?
H2 + oxidized coenzyme F0
reduced coenzyme F20
cofactor F0 i.e. 7,8-didemethy1-8-hydroxy-S-deazaflavin
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-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
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-
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
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-
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
-
-
-
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
-
-
-
?
H2 + oxidized methyl viologen
reduced methyl viologen
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-
-
?
H2 + oxidized methyl viologen
reduced methyl viologen
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-
-
?
additional information
?
-
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generation of F420H2 by Fpo with electrons derived from DIET requires an abundance of reduced methanophenazine
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-
-
additional information
?
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reduces tetrazolium dyes with molecular oxygen
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-
?
additional information
?
-
B6YTV8; B6YTV9; B6YTW0
the endogenous frhAGB-encoded hydrogenase does not exhibit F420-reducing activity
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-
-
additional information
?
-
B6YTV8; B6YTV9; B6YTW0 AND
the endogenous frhAGB-encoded hydrogenase does not exhibit F420-reducing activity
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
H2 + thioredoxin
reduced thioredoxin
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
the frhAGB-encoded hydrogenase can transfer electrons derived from oxidation of H2 to a protein target by direct contact without the involvement of an electron carrier
-
-
?
additional information
?
-
-
generation of F420H2 by Fpo with electrons derived from DIET requires an abundance of reduced methanophenazine
-
-
-
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
r
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
two alternative pathways, the F420-reducing hydrogenase and the Hmd-Mtd cycle (2-dependent methylenetetrahydromethanopterin dehydrogenase (Hmd) and F420H2-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) together reduce F420 with H2) can function in vivo for the reduction of F420 with H2. Furthermore, during growth on formate the same pathways function in reverse to produce H2 from F420H2. The lack of growth differences between the wild-type and mutant strains on H2 and CO2 suggests that neither pathway for F420 reduction is rate limiting. However, in nature the F420-reducing hydrogenase may constitute the major pathway when sufficient nickel is present, while the Hmd-Mtd cycle may be important when nickel is limiting
-
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
Methanosarcina barkeri Fusaro / DSM 804
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
enzyme of methanogenesis pathway
-
?
H2 + coenzyme F420
reduced coenzyme F420
-
enzyme of methanogenesis pathway
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
-
-
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
-
-
-
-
?
H2 + oxidized coenzyme F420
reduced coenzyme F420
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
-
-
-
?
H2 + oxidized methyl viologen
reduced methyl viologen
-
-
-
?
H2 + oxidized methyl viologen
reduced methyl viologen
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Fe-S center
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
the beta-subunit (FrhG) contains three [4Fe-4S] clusters, while the gamma-subunit (FrhB) has one [4Fe-4S] cluster
FAD
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
prosthetic group
FAD
-
bound to protein, depleted by hydrophobic interaction chromatography
FAD
-
FAD depleted enzyme reduces methyl viologen but not F420
FAD
-
0.8-0.9 mol per mol 115000 Da species, bound to gamma subunit
FAD
1 mol of the 198000 Da enzyme form contains 2 mol of FAD
additional information
-
no activity with FMN after FAD depletion
-
additional information
-
no activity with FMN after FAD depletion
-
additional information
B6YTV8; B6YTV9; B6YTW0
the alpha-subunit (FrhA) contains a binuclear [Ni-Fe] center
-
additional information
B6YTV8; B6YTV9; B6YTW0 AND
the alpha-subunit (FrhA) contains a binuclear [Ni-Fe] center
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
the alpha-subunit (FrhA) contains a binuclear [Ni-Fe] center, the beta-subunit (FrhG) contains three [4Fe-4S] clusters, while the gamma-subunit (FrhB) has one [4Fe-4S] cluster
Iron
KCl
increases activity, maximal activity at 250 mM
Ni2+
B6YTV8; B6YTV9; B6YTW0, B6YTV8; B6YTV9; B6YTW0 AND
the alpha-subunit (FrhA) contains a binuclear [Ni-Fe] center
Nickel
selenium
sulfur
Iron
1 mol of the 198000 Da enzyme form contains 28-32 mol of non-heme iron
Nickel
-
Ni3+ found in aerobically purified inactive enzyme
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
CO
-
dead end inhibitor, competitive for H2, uncompetetitive versus F0
reduced coenzyme F0
-
noncompetitive product inhibition
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