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Literature summary for 1.11.2.1 extracted from
- Functional expression of two unusual acidic peroxygenases from Candolleomyces aberdarensis in yeasts by adopting evolved secretion mutations (2021), Appl. Environ. Microbiol., 87, 1-14 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae and Pichia pastoris X-33 cells | Candolleomyces aberdarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.107 | - |
H2O2 | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis |  |
| 0.143 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 0.201 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 0.354 | - |
5-nitro-1,3-benzodioxole | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 0.357 | - |
2,6-dimethoxyphenol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 0.397 | - |
2,6-dimethoxyphenol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 0.411 | - |
H2O2 | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 0.434 | - |
5-nitro-1,3-benzodioxole | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 1.277 | - |
veratryl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 2.259 | - |
benzyl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 4.006 | - |
veratryl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 6.675 | - |
benzyl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Candolleomyces aberdarensis | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candolleomyces aberdarensis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 9% glycosylation degree | Candolleomyces aberdarensis |
| glycoprotein | mutant S61A/L79I/A252L of isoform UPO-I has 14% glycosylation degree | Candolleomyces aberdarensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 75-pg gel filtration | Candolleomyces aberdarensis |
| ammonium sulfate precipitation, phenyl Sepharose column chromatography, Mono Q column chromatography, Q Sepharose column chromatography, and Superdex 75-pg gel filtration | Candolleomyces aberdarensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 82.9 | - |
isoform UPO-II, with veratryl alcohol as substrate, at pH 6.0 and 25°C | Candolleomyces aberdarensis |
| 205.9 | - |
isoform UPO-II, with veratryl alcohol as substrate, at pH 7.0 and 25°C | Candolleomyces aberdarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Candolleomyces aberdarensis | ? | - |
? | |
| 2,6-dimethoxyphenol + H2O2 | - |
Candolleomyces aberdarensis | ? | - |
? | |
| 5-nitro-1,3-benzodioxole + H2O2 | - |
Candolleomyces aberdarensis | ? | - |
? | |
| benzyl alcohol + H2O2 | - |
Candolleomyces aberdarensis | benzaldehyde + H2O | - |
? | |
| veratryl alcohol + H2O2 | - |
Candolleomyces aberdarensis | veratraldehyde + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Candolleomyces aberdarensis |
| ? | x * 45000, SDS-PAGE | Candolleomyces aberdarensis |
| ? | x * 36500, calculated from amino acid sequence | Candolleomyces aberdarensis |
| ? | x * 37700, calculated from amino acid sequence | Candolleomyces aberdarensis |
| ? | x * 41247, MALDI-TOF mass spectrometry | Candolleomyces aberdarensis |
| ? | x * 42491, MALDI-TOF mass spectrometry | Candolleomyces aberdarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UPO-I | isoform | Candolleomyces aberdarensis |
| UPO-II | isoform | Candolleomyces aberdarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
mutant S61A/L79I/A252L of isoform UPO-I retains 50% of its activity after 10 min incubation at 56°C | Candolleomyces aberdarensis |
| 61 | - |
isoform UPO-II retains 50% of its activity after 10 min incubation at 61°C | Candolleomyces aberdarensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8 | veratryl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 22 | - |
5-nitro-1,3-benzodioxole | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 27 | - |
2,6-dimethoxyphenol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 29 | - |
5-nitro-1,3-benzodioxole | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 33 | - |
H2O2 | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 87 | - |
2,6-dimethoxyphenol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 97 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 106 | - |
veratryl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 114 | - |
H2O2 | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 117 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 214 | - |
benzyl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 254 | - |
benzyl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
isoform UPO-II | Candolleomyces aberdarensis |
| 6 | - |
mutant S61A/L79I/A252L of isoform UPO-I | Candolleomyces aberdarensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | mutant S61A/L79I/A252L of isoform UPO-I retains over 40% of its relative activity in the pH range from 3.0 to 9.0 | Candolleomyces aberdarensis |
| 4 | 7 | isoform UPO-II retains over 40% of its relative activity in the pH range from 4.0 to 7.0 | Candolleomyces aberdarensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Candolleomyces aberdarensis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Candolleomyces aberdarensis | calculated from amino acid sequence | - |
5.1 |
| Candolleomyces aberdarensis | calculated from amino acid sequence | - |
5.3 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22 | - |
veratryl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 26 | - |
veratryl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 32 | - |
benzyl alcohol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 62 | - |
5-nitro-1,3-benzodioxole | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 67 | - |
5-nitro-1,3-benzodioxole | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 76 | - |
2,6-dimethoxyphenol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 112 | - |
benzyl alcohol | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 219 | - |
2,6-dimethoxyphenol | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 277 | - |
H2O2 | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
| 308 | - |
H2O2 | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 582 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant S61A/L79I/A252L of isoform UPO-I, at pH 6.0 and 25°C | Candolleomyces aberdarensis | |
| 679 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | isoform UPO-II, at pH 7.0 and 25°C | Candolleomyces aberdarensis | |
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Release 2023.1 (January 2023)
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