Literature summary for 1.11.2.1 extracted from

Linde, D.; Olmedo, A.; Gonzalez-Benjumea, A.; Estevez, M.; Renau-Minguez, C.; Carro, J.; Fernandez-Fueyo, E.; Gutierrez, A.; Martinez, A.
Two new unspecific peroxygenases from heterologous expression of fungal genes in Escherichia coli (2020), Appl. Environ. Microbiol., 86, e02899-19 .

Search for more literature for 1.11.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli C41 cells	Achaetomiella virescens
expressed in Escherichia coli C41 cells	Daldinia caldariorum

Protein Variants

Protein Variants	Comment	Organism
F88L	the mutation strongly increases the enzyme's catalytic efficiency in oxidizing veratryl alcohol (21fold), benzyl alcohol (11fold), and naphthalene (5fold) compared to the wild type enzyme	Achaetomiella virescens
T158F	the variant improves the oxidation only of benzyl alcohol but decreases the oxidation of veratryl alcohol and naphthalene compared with the wild type enzyme	Achaetomiella virescens

Inhibitors

Inhibitors	Comment	Organism	Structure
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	-	Achaetomiella virescens
benzyl alcohol	-	Daldinia caldariorum
veratryl alcohol	-	Daldinia caldariorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.059	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
0.16	-	veratryl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
0.179	-	benzyl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
0.19	-	Naphthalene	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.239	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.255	-	Naphthalene	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.332	-	Naphthalene	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
0.337	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.337	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.45	-	Naphthalene	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.623	-	benzyl alcohol	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
1.15	-	veratryl alcohol	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
1.87	-	benzyl alcohol	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
2.94	-	veratryl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens
3.97	-	veratryl alcohol	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
7.1	-	benzyl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	gel filtration	Achaetomiella virescens
55000	-	gel filtration	Daldinia caldariorum

Organism

Organism	UniProt	Comment	Textmining
Achaetomiella virescens	-	synonym Chaetomium virescens	-
Daldinia caldariorum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap ion-exchange column chromatography and Superdex 75 gel filtration	Achaetomiella virescens
HiTrap ion-exchange column chromatography and Superdex 75 gel filtration	Daldinia caldariorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2	-	Achaetomiella virescens	oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O	-	?
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2	-	Daldinia caldariorum	oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O	-	?
4-nitrobenzaldehyde + H2O2	-	Achaetomiella virescens	4-nitrobenzoic acid + H2O	-	?
4-nitrobenzaldehyde + H2O2	-	Daldinia caldariorum	4-nitrobenzoic acid + H2O	-	?
benzyl alcohol + H2O2	-	Achaetomiella virescens	benzaldehyde + H2O	-	?
benzyl alcohol + H2O2	-	Daldinia caldariorum	benzaldehyde + H2O	-	?
naphthalene + H2O2	-	Achaetomiella virescens	?	-	?
naphthalene + H2O2	-	Daldinia caldariorum	?	-	?
veratryl alcohol + H2O2	-	Achaetomiella virescens	veratraldehyde + H2O	-	?
veratryl alcohol + H2O2	-	Daldinia caldariorum	veratraldehyde + H2O	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 27000, SDS-PAGE	Daldinia caldariorum
homodimer	2 * 24000, SDS-PAGE	Achaetomiella virescens
homodimer	2 * 29609, calculated from amino acid sequence	Daldinia caldariorum
homodimer	2 * 29616, calculated from amino acid sequence	Achaetomiella virescens

Synonyms

Synonyms	Comment	Organism
UPO	-	Achaetomiella virescens
UPO	-	Daldinia caldariorum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.17	-	Naphthalene	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
1.08	-	Naphthalene	at 25°C, pH not specified in the publication	Achaetomiella virescens
1.56	-	veratryl alcohol	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
2.24	-	veratryl alcohol	at 25°C, pH not specified in the publication	Achaetomiella virescens
2.3	-	Naphthalene	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
2.39	-	veratryl alcohol	at 25°C, pH not specified in the publication	Daldinia caldariorum
6.73	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	at 25°C, pH not specified in the publication	Daldinia caldariorum
11.24	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
16.09	-	benzyl alcohol	at 25°C, pH not specified in the publication	Daldinia caldariorum
18.6	-	veratryl alcohol	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
22.1	-	Naphthalene	at 25°C, pH not specified in the publication	Daldinia caldariorum
62.9	-	benzyl alcohol	at 25°C, pH not specified in the publication	Achaetomiella virescens
63.7	-	benzyl alcohol	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
126.3	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
153.8	-	benzyl alcohol	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
157.2	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	at 25°C, pH not specified in the publication	Achaetomiella virescens

Cofactor

Cofactor	Comment	Organism	Structure
heme	the enzyme contains about 0.5 mol of heme per mole of protein	Achaetomiella virescens
heme	the enzyme contains about 0.5 mol of heme per mole of protein	Daldinia caldariorum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
7.86	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	at 25°C, pH not specified in the publication	Achaetomiella virescens
9.28	-	benzyl alcohol	at 25°C, pH not specified in the publication	Daldinia caldariorum
45.4	-	veratryl alcohol	at 25°C, pH not specified in the publication	Daldinia caldariorum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.38	-	veratryl alcohol	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.68	-	Naphthalene	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
0.75	-	veratryl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens
2.42	-	Naphthalene	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens
8.9	-	benzyl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens
12.2	-	Naphthalene	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
14.8	-	veratryl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
16.1	-	veratryl alcohol	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
32.1	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
66.8	-	Naphthalene	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
82.4	-	benzyl alcohol	mutant enzyme T158F, at 25°C, pH not specified in the publication	Achaetomiella virescens
90	-	benzyl alcohol	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
102.1	-	benzyl alcohol	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
114.4	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	wild type enzyme, at 25°C, pH not specified in the publication	Daldinia caldariorum
375	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	mutant enzyme F88L, at 25°C, pH not specified in the publication	Achaetomiella virescens
656	-	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate)	wild type enzyme, at 25°C, pH not specified in the publication	Achaetomiella virescens

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Release 2023.1 (January 2023)