Cloned (Comment) | Organism |
---|---|
- |
Sarocladium strictum |
Crystallization (Comment) | Organism |
---|---|
- |
Sarocladium strictum |
Protein Variants | Comment | Organism |
---|---|---|
C130A | FAD binding site, FAD covalently attached | Sarocladium strictum |
H70A | FAD binding site, FAD covalently attached | Sarocladium strictum |
H70A/C130A | no activity, lack of essential FAD cofactor | Sarocladium strictum |
Y310A | larger amount of carbohydrates | Sarocladium strictum |
General Stability | Organism |
---|---|
and wild-type proteins are 1.9 M, 2.4 M, 2.6 M, and 3.2 M, respectively. | Sarocladium strictum |
and wild-type proteins are 48°C, 52°C, 46°C, and 56°C, respectively. | Sarocladium strictum |
The melting temperatures for the H70A, C130A, H70A/C130A, | Sarocladium strictum |
The [guanidine HCl]1/2 values for the H70A, C130A, H70A/C130A, | Sarocladium strictum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
cellobiose | wild type protein | Sarocladium strictum | |
0.22 | - |
cellobiose | H70A mutant protein | Sarocladium strictum | |
0.4 | - |
cellobiose | C130A mutant protein | Sarocladium strictum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sarocladium strictum | Q6PW77 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Sarocladium strictum |
Purification (Comment) | Organism |
---|---|
- |
Sarocladium strictum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cello-oligosaccharide + acceptor | - |
Sarocladium strictum | ? | - |
? | |
cellobiose + acceptor | - |
Sarocladium strictum | ? | - |
? | |
D-glucose + acceptor | - |
Sarocladium strictum | ? | - |
? | |
lactose + acceptor | - |
Sarocladium strictum | ? | - |
? | |
malto-oligosaccharide + acceptor | - |
Sarocladium strictum | ? | - |
? | |
maltose + acceptor | - |
Sarocladium strictum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucooligosaccharide oxidase | - |
Sarocladium strictum |
GOOX | - |
Sarocladium strictum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
cellobiose | H70A mutant protein | Sarocladium strictum | |
0.58 | - |
cellobiose | C130A mutant protein | Sarocladium strictum | |
11.9 | - |
cellobiose | wild type protein | Sarocladium strictum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Sarocladium strictum |