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Information on EC 1.1.99.B3 - glucooligosaccharide oxidase
for references in articles please use BRENDA:EC1.1.99.B3preliminary BRENDA-supplied EC number
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EC Tree
1.1.99.B3 glucooligosaccharide oxidaseSpecify your search results
Word Map
- 1.1.99.B3
- strictum
-
acremonium
- fad
-
sarocladium
- xylo-oligosaccharides
- maltose
- cellobiose
- maltooligosaccharides
-
cello
-
carbohydrate-binding
- isoalloxazine
-
flavinylation
-
flavoenzyme
-
xylos
- cello-oligosaccharide
- maltotriose
-
6-s-cysteinyl
- maltopentaose
-
lactobionic
- xylobiose
-
hardwood
- bran
- maltohexaose
- thermocellum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
glucooligosaccharide oxidase, goox-vn, gluco-oligosaccharide oxidase, carbohydrate:acceptor oxidoreductase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
gluco-oligosaccharide oxidase
GOOX
GOOX-VN
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[beta-D-hexosyl-(1->4)]n-beta-D-hexose + acceptor = [beta-D-hexosyl-(1->4)]n-D-hexono-1,5-lactone + reduced acceptor
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
carbohydrate:acceptor oxidoreductase
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
6-deoxy-D-glucose + 2,6-dichlorophenol indophenol
?
-
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
beta-D-glucose + 2,6-dichlorophenol indophenol
D-glucono-1,5-lactone + ?
-
DCIP, electron acceptor specificity determined
-
-
?
beta-D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
kinetic parameters for various sugars shown, enzyme properties compared to other sugar oxidizing enzymes
-
-
?
cellobiose + 2,6-dichlorophenol indophenol
?
-
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
cellohexaose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
Glc-beta-(1->4)-Glc-beta-(1->4)-D-glucono-1,5-lactone + H2O2
-
-
-
?
D-galactose + 2,6-dichlorophenol indophenol
?
-
hexoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
D-glucose + O2
? + H2O2
highest catalytic efficiency with D-glucose
-
-
?
D-xylose + 2,6-dichlorophenol indophenol
?
-
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
L-arabinose + 2,6-dichlorophenol indophenol
?
-
pentoses as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
lactose + 2,6-dichlorophenol indophenol
?
-
substrate specificity for alpha-lactose and beta-lactose determined
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-
?
lactose + O2
?
-
substrate specificity for alpha-lactose and beta-lactose determined
-
-
?
maltose + 2,6-dichlorophenol indophenol
?
-
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
maltose + O2
4-O-alpha-D-glucopyranosyl-D-glucono-1,5-lactone + H2O2
-
-
-
?
xylobiose + 2,6-dichlorophenol indophenol
?
-
disaccharides as substrates determined, 2,6-dichlorophenol indophenol as electron acceptor
-
-
?
xylobiose + O2
xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylohexaose + O2
D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylopentaose + O2
D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-Xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
xylotriose + O2
xyl-beta-(1->4)-xyl-beta-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
-
-
?
cellobiose + O2
cellobiono-1,5-lactone + H2O2
-
-
-
?
cellohexaose + O2
cellohexaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellopentaose + O2
cellopentaono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
cellotetraono-1,5-lactone + H2O2
-
-
-
?
cellotetraose + O2
cellotetraono-1,5-lactone + H2O2
-
-
-
?
cellotriose + O2
cellotriono-1,5-lactone + H2O2
-
-
-
?
D-glucose + O2
D-glucono-1,5-lactone + H2O2
-
-
-
?
xylobiose + O2
xylobiono-1,5-lactone + H2O2
highest activity
-
-
?
xylobiose + O2
xylobiono-1,5-lactone + H2O2
highest activity
-
-
?
additional information
?
-
enzyme GOOX oxidizes the anomeric carbon of carbohydrate substrates through an FAD reduction mechanism. The enzyme displays a comparatively broad substrate profile, ranging from D-xylose, and galactose, to malto-, cello- and xylooligosaccharides. GOOX oxidizes oligosaccharides more effectively than monosaccharides
-
-
?
additional information
?
-
no activity with mannose
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-glucose + O2
? + H2O2
highest catalytic efficiency with D-glucose
-
-
?
additional information
?
-
no activity with mannose
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
the FAD cofactor is bi-covalently linked through cysteine and histidine residues
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
mutants Y300D and CtCBM22A_Y300A GOOX variants show no H2O2 inactivation on cellobiose
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.078 - 0.09
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
75
6-deoxy-D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.078
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.09
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.052
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.059
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.08
cellobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.06
cellohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.31
cellohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.03
cellopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor