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Literature summary for 1.1.99.18 extracted from

  • Gangwar, R.; Rasool, S.; Mishra, S.
    Purified cellobiose dehydrogenase of Termitomyces sp. OE147 fuels cellulose degradation resulting in the release of reducing sugars (2021), Prep. Biochem. Biotechnol., 51, 488-496 .
    View publication on PubMed
Search for more literature for 1.1.99.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 cellobiose pH 5.0, 25°C Termitomyces sp. OE 147
16.55
-
 lactose pH 5.0, 25°C Termitomyces sp. OE 147
339.2
-
 maltose pH 5.0, 25°C Termitomyces sp. OE 147

Metals/Ions

Metals/Ions Comment Organism Structure
FeCl3 accelerates activity during the early stages of incubation Termitomyces sp. OE 147

Organism

Organism UniProt Comment Textmining
Termitomyces sp. OE 147
-
-
-

Purification (Commentary)

Purification (Comment) Organism
172fold purification, combination of ultrafiltration, ion-exchange and gel-filtration chromatography Termitomyces sp. OE 147

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
324
-
 pH 5.0, 25°C, substrate: lactose Termitomyces sp. OE 147

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol
-
 Termitomyces sp. OE 147 cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
lactose + 2,6-dichloroindophenol 80% of the activity with cellobiose Termitomyces sp. OE 147 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
maltose + 2,6-dichloroindophenol 28% of the activity with cellobiose Termitomyces sp. OE 147 4-0-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?

Synonyms

Synonyms Comment Organism
Cdh
-
 Termitomyces sp. OE 147

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Termitomyces sp. OE 147

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 85 30°C: about 70% of maximal activity, 85°C: about 85% of maximal activity Termitomyces sp. OE 147

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 1 h, stable Termitomyces sp. OE 147
35
-
 1 h, about 10% loss of activity Termitomyces sp. OE 147
45
-
 1 h, about 15% loss of activity Termitomyces sp. OE 147
55
-
 1 h, about 30% loss of activity Termitomyces sp. OE 147

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.52
-
 maltose pH 5.0, 25°C Termitomyces sp. OE 147
59.56
-
 cellobiose pH 5.0, 25°C Termitomyces sp. OE 147
66.95
-
 lactose pH 5.0, 25°C Termitomyces sp. OE 147

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Termitomyces sp. OE 147

pH Range

pH Minimum pH Maximum Comment Organism
3 6 pH 3.0: about 50% of maximal activity, pH 7.0: about 75% of maximal activity Termitomyces sp. OE 147

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 3 h, about 20% loss of activity Termitomyces sp. OE 147
5 6 3 h, less than 5% loss of activity Termitomyces sp. OE 147
7
-
 3 h, about 10% loss of activity Termitomyces sp. OE 147
8
-
 2 h, about 40% loss of activity Termitomyces sp. OE 147
9
-
 2 h, about 60% loss of activity Termitomyces sp. OE 147

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 maltose pH 5.0, 25°C Termitomyces sp. OE 147
4.05
-
 lactose pH 5.0, 25°C Termitomyces sp. OE 147
397
-
 cellobiose pH 5.0, 25°C Termitomyces sp. OE 147