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Literature summary for 1.1.99.18 extracted from

  • Turbe-Doan, A.; Arfi, Y.; Record, E.; Estrada-Alvarado, I.; Levasseur, A.
    Heterologous production of cellobiose dehydrogenases from the basidiomycete Coprinopsis cinerea and the ascomycete Podospora anserina and their effect on saccharification of wheat straw (2013), Appl. Microbiol. Biotechnol., 97, 4873-4885.
    View publication on PubMed
Search for more literature for 1.1.99.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus niger Coprinopsis cinerea
expressed in Aspergillus niger Podospora anserina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
 cellotriose at 6.0 pH and 30 °C Podospora anserina
0.041
-
 cellotetraose at 6.0 pH and 30 °C Podospora anserina
0.043
-
 lactose at 6.0 pH and 30 °C Podospora anserina
0.132
-
 cellobiose at 6.0 pH and 30 °C Podospora anserina
0.156
-
 cellobiose at 5.0 pH and 30 °C Coprinopsis cinerea
0.281
-
 cellotetraose at 5.0 pH and 30 °C Coprinopsis cinerea
0.352
-
 cellotriose at 5.0 pH and 30 °C Coprinopsis cinerea
1.523
-
 lactose at 5.0 pH and 30 °C Coprinopsis cinerea
328.6
-
 D-glucose at 6.0 pH and 30 °C Podospora anserina
354.4
-
 D-glucose at 5.0 pH and 30 °C Coprinopsis cinerea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 x * 83000, SDS-PAGE Coprinopsis cinerea
83000
-
 x * 83000, SDS-PAGE Podospora anserina

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-
Podospora anserina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chelating Sepharose column chromatography Coprinopsis cinerea
chelating Sepharose column chromatography Podospora anserina

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Coprinopsis cinerea
-
mycelium
-
 Podospora anserina
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
 Coprinopsis cinerea cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellobiose + 2,6-dichlorophenolindophenol
-
 Podospora anserina cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellotetraose + 2,6-dichlorophenolindophenol
-
 Coprinopsis cinerea cellotetraono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellotetraose + 2,6-dichlorophenolindophenol
-
 Podospora anserina cellotetraono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellotriose + 2,6-dichlorophenolindophenol
-
 Coprinopsis cinerea cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellotriose + 2,6-dichlorophenolindophenol
-
 Podospora anserina cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Coprinopsis cinerea D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Podospora anserina D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + 2,6-dichlorophenolindophenol
-
 Coprinopsis cinerea lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + 2,6-dichlorophenolindophenol
-
 Podospora anserina lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 83000, SDS-PAGE Coprinopsis cinerea
? x * 83000, SDS-PAGE Podospora anserina

Synonyms

Synonyms Comment Organism
Cdh
-
 Coprinopsis cinerea
Cdh
-
 Podospora anserina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Coprinopsis cinerea
65 70
-
 Podospora anserina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme is thermostable at 30°C and loses approximately 20-25% of its activity after 4 h at 40°C and half of its activity after 17 min at 50°C Podospora anserina
30 50 the enzyme is thermostable at 30°C and loses approximately 20-25% of its activity after 4 h at 40°C and half of its activity after 30 min at 50°C Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
 D-glucose at 6.0 pH and 30 °C Podospora anserina
2.6
-
 lactose at 6.0 pH and 30 °C Podospora anserina
3.8
-
 cellotriose at 6.0 pH and 30 °C Podospora anserina
4
-
 cellotetraose at 6.0 pH and 30 °C Podospora anserina
8.56
-
 cellobiose at 6.0 pH and 30 °C Podospora anserina
34.4
-
 D-glucose at 5.0 pH and 30 °C Coprinopsis cinerea
96.9
-
 cellobiose at 5.0 pH and 30 °C Coprinopsis cinerea
101.6
-
 cellotetraose at 5.0 pH and 30 °C Coprinopsis cinerea
136.3
-
 lactose at 5.0 pH and 30 °C Coprinopsis cinerea
156
-
 cellotriose at 5.0 pH and 30 °C Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Coprinopsis cinerea
6
-
-
 Podospora anserina

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 the enzyme retains its activity after 2 and 24 h at pH 7.0 Coprinopsis cinerea
7
-
 the enzyme retains its activity after 2 and 24 h at pH 7.0 Podospora anserina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00494
-
 D-glucose at 6.0 pH and 30 °C Podospora anserina
0.097
-
 D-glucose at 5.0 pH and 30 °C Coprinopsis cinerea
60
-
 lactose at 6.0 pH and 30 °C Podospora anserina
64
-
 cellobiose at 6.0 pH and 30 °C Podospora anserina
89.4
-
 lactose at 5.0 pH and 30 °C Coprinopsis cinerea
99
-
 cellotetraose at 6.0 pH and 30 °C Podospora anserina
142
-
 cellotriose at 6.0 pH and 30 °C Podospora anserina
361
-
 cellotetraose at 5.0 pH and 30 °C Coprinopsis cinerea
442
-
 cellotriose at 5.0 pH and 30 °C Coprinopsis cinerea
621
-
 cellobiose at 5.0 pH and 30 °C Coprinopsis cinerea