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Literature summary for 1.1.99.18 extracted from

  • Karapetyan, K.N.; Yachkova, S.N.; Vasil‘chenko, L.G.; Borzykh, M.N.; Rabinovich, M.L.
    Isolation and characterization of a cellobiose dehydrogenase formed by a nonsporulating mycelial fungus INBI 2-26(-) (2003), Appl. Biochem. Microbiol., 39, 564-572.
    View publication on PubMed
Search for more literature for 1.1.99.18

General Stability

General Stability Organism
enzyme reduced by cellobiose is more stable than the nonreduced form Fungi
presence of 2,6-dichlorophenol indophenol decreases stability eight times at pH 6.0 Fungi

Inhibitors

Inhibitors Comment Organism Structure
gentiobiose 1 mM, 22% inhibition of reaction with lactose, 0.00625 mM Fungi
methyl-beta-D-cellobioside 1 mM, 40% inhibition of reaction with lactose, 0.00625 mM Fungi
NaN3 0.5 mM, 15% inhibition of reaction with lactose, 0.0125 mM Fungi
tri-N-acetylchitotriose 0.5 mM, 20% inhibition of reaction with lactose, 0.00625 mM Fungi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00467
-
 cellobiose pH 6.0, 20°C Fungi
0.00476
-
 cellobiose pH 4.0, 20°C Fungi
0.00496
-
 cellobiose pH 8.0, 20°C Fungi
0.012
-
 2,6-dichlorophenol indophenol pH 4.0, 20°C Fungi
0.0158
-
 cytochrome c pH 6.0, 20°C Fungi
0.016
-
 2,6-dichlorophenol indophenol pH 6.0, 20°C Fungi
0.017
-
 cytochrome c pH 4.0, 20°C Fungi
0.0305
-
 cytochrome c pH 8.0, 20°C Fungi
0.056
-
 lactose pH 6.0, 20°C Fungi
0.089
-
 2,6-dichlorophenol indophenol pH 8.0, 20°C Fungi

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Fungi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaF 50 mM, activation of reaction with lactose (0.0125 mM) to 120% of control Fungi
NaN3 1 mM, activation of reaction with lactose (0.0125 mM) to 110% of control Fungi

Organism

Organism UniProt Comment Textmining
Fungi
-
 INBI-2-26(-), mycelial sterilia, nonsporulating, optimal growth temperature 28°C, isolated from dioxin-containing tropical soils (South Vietnam)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fungi

Source Tissue

Source Tissue Comment Organism Textmining
culture medium fungi grown in medium supplemented by a source of cellulose Fungi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.78
-
-
 Fungi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 2,6-dichlorophenol indophenol 20% of the activity with lactose Fungi 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
beta-maltose + 2,6-dichlorophenol indophenol 20% of the activity with lactose Fungi 4-O-beta-glucosyl-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellobiose + 2,6-dichlorophenol indophenol
-
 Fungi cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellobiose + cytochrome c
-
 Fungi cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
D-glucose + 2,6-dichlorophenol indophenol 27% of the activity with lactose Fungi D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
lactose + 2,6-dichlorophenol indophenol
-
 Fungi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 6.0, 50% loss of activity after 99 min Fungi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 cytochrome c pH 4.0, 20°C Fungi
4
-
 cellobiose pH 8.0, 20°C Fungi
6.4
-
 2,6-dichlorophenol indophenol pH 4.0, 20°C Fungi
7.6
-
 2,6-dichlorophenol indophenol pH 8.0, 20°C Fungi
7.9
-
 cellobiose pH 4.0, 20°C Fungi
8.1
-
 cytochrome c pH 6.0, 20°C Fungi
8.9
-
 cellobiose pH 6.0, 20°C Fungi
11.3
-
 cytochrome c pH 8.0, 20°C Fungi
11.3
-
 2,6-dichlorophenol indophenol pH 6.0, 20°C Fungi
14
-
 lactose pH 6.0, 20°C Fungi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 reaction with 2,6-dichlorophenol indophenol Fungi
6
-
 reaction with cytochrome c Fungi

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with 2,6-dichlorophenol indophenol Fungi

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 55°C, 50% loss of activity after 99 min Fungi