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Literature summary for 1.1.3.6 extracted from

  • Alapati, K.; Handanahal, S.
    Characterization of cholesterol oxidase from a marine Streptomyces sp. and its cytotoxicity (2020), Process Biochem., 89, 175-185 .
No PubMed abstract available
Search for more literature for 1.1.3.6

Application

Application Comment Organism
drug development the enzyme showed significant cytotoxicity on breast (MCF-7), nasopharyngeal (KB) and ovarian (OVCAR) cancer cell lines at very low concentrations ranging from 0.000093 to 0.00014 mM. It exhibits relatively less cytotoxicity on primary mouse embryonic fibroblast (3T3) cells. Thus, cholesterol oxidase from Streptomyces sp. AKHSS could be a potential anticancer agent Streptomyces sp. AKHSS

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Streptomyces sp. AKHSS

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 cholesterol pH 9.0, 37°C Streptomyces sp. AKHSS

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound, not secreted Streptomyces sp. AKHSS 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgSO4 stimulates the enzyme activity Streptomyces sp. AKHSS
ZnSO4 stimulates the enzyme activity Streptomyces sp. AKHSS

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42840
-
 electron-spray ionization mass spectrometry Streptomyces sp. AKHSS
48000
-
 SDS-PAGE Streptomyces sp. AKHSS

Organism

Organism UniProt Comment Textmining
Streptomyces sp. AKHSS
-
-
-

Purification (Commentary)

Purification (Comment) Organism
polyethylene glycol 4000 precipitation, diethylaminoethyl Sephacel anionic column chromatography and Superdex-200 gel filtration Streptomyces sp. AKHSS

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Streptomyces sp. AKHSS cholest-5-en-3-one + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Streptomyces sp. AKHSS

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, stable up to Streptomyces sp. AKHSS

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Streptomyces sp. AKHSS