Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | stimulates at concentration up to 0.2% | Pimelobacter simplex |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Pimelobacter simplex | |
sulfhydryl reagents | - |
Pimelobacter simplex | |
Triton X-100 | stimulates at concentration up to 0.2% | Pimelobacter simplex |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Pimelobacter simplex | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pimelobacter simplex | - |
- |
- |
Purification (Comment) | Organism |
---|---|
inducible and constitutive enzyme | Pimelobacter simplex |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Pimelobacter simplex |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pimelobacter simplex | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.42 | - |
inducible enzyme | Pimelobacter simplex |
3.65 | - |
constitutive enzyme | Pimelobacter simplex |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Pimelobacter simplex | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pimelobacter simplex | cholest-5-en-3-one + H2O2 | - |
? | |
ergosterol + O2 | low activity | Pimelobacter simplex | dehydroergosterol + H2O2 | - |
? | |
stigmasterol + O2 | - |
Pimelobacter simplex | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pimelobacter simplex |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | 60 | constitutive enzyme, 50% of maximal activity at 33°C, 70% at 60°C, inducible enzyme, 50% of maximal activity at 33°C and 60°C | Pimelobacter simplex |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pimelobacter simplex |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | inducible enzyme, 80% of maximal activity at pH 6.0, 50% at pH 9.5, constitutive enzyme, 70% of maximal activity at pH 6.0, 50% at pH 9.5 | Pimelobacter simplex |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 30°C, 2 h, stable | Pimelobacter simplex |