Literature summary for 1.1.3.46 extracted from

Lyu, S.; Lin, K.; Yeh, H.; Li, Y.; Huang, C.; Wang, Y.; Shih, H.; Hsu, N.; Wu, C.; Li, T.
The flavin mononucleotide cofactor in alpha-hydroxyacid oxidases exerts its electrophilic/nucleophilic duality in control of the substrate-oxidation level (2019), Acta Crystallogr. Sect. D, 75, 918-929 .

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Amycolatopsis orientalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of wild-type and mutant Y128F with and without substrate. Cofactor FMNox possesses an electrophilic/nucleophilic duality. In the Y128F mutant the active-site perturbation ensemble facilitates the polarization of FMNox to a nucleophilic ylide, which is in a position to act on an alpha-ketoacid, forming an N5-acyl-FMNred dead-end adduct. In four-electron oxidation of mutant Y128F, an intramolecular disproportionation reaction via an N5-alkanol-FMNred C'alpha carbanion intermediate may account for the ThDP/PLP/NADPH-independent oxidative decarboxylation reaction	Amycolatopsis orientalis

Crystallization (Comment)

Organism

structures of wild-type and mutant Y128F with and without substrate. Cofactor FMNox possesses an electrophilic/nucleophilic duality. In the Y128F mutant the active-site perturbation ensemble facilitates the polarization of FMNox to a nucleophilic ylide, which is in a position to act on an alpha-ketoacid, forming an N5-acyl-FMNred dead-end adduct. In four-electron oxidation of mutant Y128F, an intramolecular disproportionation reaction via an N5-alkanol-FMNred C'alpha carbanion intermediate may account for the ThDP/PLP/NADPH-independent oxidative decarboxylation reaction

Amycolatopsis orientalis

Protein Variants

Protein Variants	Comment	Organism
Y128F	mutant is capable of oxidizing mandelate to benzoate via a four-electron oxidative decarboxylation reaction	Amycolatopsis orientalis

Organism

Organism	UniProt	Comment	Textmining
Amycolatopsis orientalis	O52792	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-mandelate + O2	-	Amycolatopsis orientalis	2-phenyl-2-oxoacetate + H2O2	-	?
2 (S)-mandelate + O2	-	Amycolatopsis orientalis	2 benzoate + 2 CO2	-	?

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Amycolatopsis orientalis

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Release 2023.1 (January 2023)