Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas mevalonii |
Protein Variants | Comment | Organism |
---|---|---|
C156/296 A | fully active, resistant to N-ethylmaleimide | Pseudomonas mevalonii |
C156A | fully active, resistant to N-ethylmaleimide | Pseudomonas mevalonii |
C296A | fully active | Pseudomonas mevalonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mevalonii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54.2 | - |
cysteine-free mutant, mevalonate acylation | Pseudomonas mevalonii |
65.6 | - |
C156A-mutant, mevalonate acylation | Pseudomonas mevalonii |
70.5 | - |
wild-type enzyme, mevalonate acylation | Pseudomonas mevalonii |
77.7 | - |
C296A-mutant, mevalonate acylation | Pseudomonas mevalonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mevalonate + CoA + 2 NAD+ | - |
Pseudomonas mevalonii | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | four-electron pyridine oxidoreductase | Pseudomonas mevalonii | |
NADH | reverse reaction | Pseudomonas mevalonii |