General Stability | Organism |
---|---|
dilute solutions, 1 mg/ml or less, precipitate upon thawing | Salmonella enterica subsp. enterica serovar Typhimurium |
slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Ca2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Co2+ | above 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
iodoacetamide | 50 mM, slight inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
iodoacetate | 50 mM, slight inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
K+ | above 1 M, slight inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
Li+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | above 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
N-ethylmaleimide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
NEM | 5 mM, 50% inhibition, not reversed by 2-mercaptoethanol | Salmonella enterica subsp. enterica serovar Typhimurium | |
p-hydroxymercuribenzoate | pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol | Salmonella enterica subsp. enterica serovar Typhimurium | |
PCMB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
(2R,3S)-3-isopropylmalate | pH 9.0 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.1 | - |
NAD+ | pH 9.0 | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
K+ | monovalent cation required, maximal activity at 0.05 M | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
NH4+ | monovalent cations required, NH4+ can partially replace K+ | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl | Salmonella enterica subsp. enterica serovar Typhimurium |
70000 | - |
equilibrium sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Salmonella enterica subsp. enterica serovar Typhimurium | penultimate step in leucine biosynthesis | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
strain leu A-124 | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
-20°C, pH 5.5-7.5, fully active | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | penultimate step in leucine biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
r | |
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxoisohexanoate + thionicotinamideNADH + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | stable if stored concentrated | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium |